El-azab, N., Mansy, A., El-Mahalaway, A., Sabry, D. (2018). Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study. test, 41(2), 150-166. doi: 10.21608/EJH.2018.13838
Nahla El-Eraky El-azab; Aisha E. Mansy; Abeer M. El-Mahalaway; Dina Sabry. "Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study". test, 41, 2, 2018, 150-166. doi: 10.21608/EJH.2018.13838
El-azab, N., Mansy, A., El-Mahalaway, A., Sabry, D. (2018). 'Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study', test, 41(2), pp. 150-166. doi: 10.21608/EJH.2018.13838
El-azab, N., Mansy, A., El-Mahalaway, A., Sabry, D. Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study. test, 2018; 41(2): 150-166. doi: 10.21608/EJH.2018.13838

Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study

Egyptian Journal of Histology
Article 3, Volume 41, Issue 2, June 2018, Pages 150-166    PDF (19.83 M)
Document Type: Original Article
DOI: 10.21608/EJH.2018.13838
Authors
Nahla El-Eraky El-azab1; Aisha E. Mansy2; Abeer M. El-Mahalaway2; Dina Sabry3
1Histology, faculty of medicine, benha university Egypt
2Department of Histology and Cell Biology, Benha Faculty of Medicine, Benha University, Egypt
3Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
therapeutic effects. Bone marrow mesenchymal stem cells (BMSCs) are a novel approach with promising therapeutic potential for curing human diseases.
Aim of the work: To evaluate the effect of pomegranate and BMSCs on experimentally induced gastric ulcer in adult male rats.
Materials and Methods: Sixty adult male rats were divided into four groups. Group I kept as the control group. Group II (Gastric ulcer), rats received 100% ethanol orally by gastric tube. Group (III) subdivided into 3 subgroups. Subgroup IIIa included rats with gastric ulcer that treated with pomegranate only. Subgroup IIIb, rats with gastric ulcer treated with BMSCs alone. Subgroup IIIc; gastric ulcer rats treated with a combination of pomegranate and BMSCs. Group IV included the untreated gastric ulcer rats. Fundus of stomach specimens were prepared and examined using histological and immunohistochemical techniques.
Results: Groups II and IV showed various changes as deep erosion, marked damage of most of the cells. The presence of the inflammatory cells in gastric mucosa were supported by a significant increase of tumor necrosis factor α (TNF-α) expression and collagen fibers depositions (P<0.01). A significant decrease of Periodic Acid Schiff (PAS) positive reaction and vascular endothelial growth factor (VEGF) expression was observed (P<0.01). Subgroup IIIa showed attenuation of some histological changes, while subgroups IIIb and IIIc revealed more improvement of the histological and immunohistochemical changes described before.
Conclusion: BMSCs can ameliorate experimentally-induced gastric injury in rats and protect the gastric tissue. Addition of pomegranate to BMSCs can lead to better results.
Keywords
Bone marrow mesenchymal stem cells (BMSCs); Gastric ulcer; pomegranate; tumor necrosis factor-α; vascular endothelial growth factor (VEGF)
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