Influence of Sudden Thermal Stresses on Growth Rate and Enzymatic Identification of Bombyx mori L. Larvae | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Article 7, Volume 6, Issue 1, June 2014, Pages 71-81 PDF (338.11 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2014.16048 | ||
| Authors | ||
| Rehab H. Taha; Saad I. A. I.; Eman M. Hassan | ||
| Sericulture Research Department (SRD), Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Egypt. | ||
| Abstract | ||
| The purpose of this study is to determine the impact of different thermal stresses for different periods on univoltine Bombyx mori embryos and grown larvae in terms of larval growth rates, effective rate of rearing and the enzymatic proliferation of larval haemolymph. Egg groups was exposed to different temperatures; 0°C for one hour, 0°C for two hours, 40°C for one hour and 40°C for two hours, followed by 2 hours recovery at room temperature in addition to a control group, the same was applied to the grown larvae. Larval haemolymph of tested groups were subjected to enzyme electrophoretic analysis by native polyacrylamide gel electrophoresis (PAGE) for phosphatases and esterases identification.Exposure to sudden thermal stress of 0°C for one hour at larval stage recorded the highest growth rate. The sudden thermal stresses did notsignificantly affect the effective rate of rearing. However, thermal stresses increased ACPase and ALKPase activities and decreased α and β esterases activities comparing to control. These results suggest applying sudden heat thermal proteins in breeding strategy for inducing robustness in productive breeds. | ||
| Keywords | ||
| Silkworm; thermal stress; egg; Larvae; growth rate; Effective rate of rearing; Enzymes | ||
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