Effect of soy isoflavones on some immunological parameters in ovariectomized female rats. | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Article 10, Volume 6, Issue 1, June 2014, Pages 101-113 PDF (612.09 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2014.16051 | ||
| Authors | ||
| Hend M. Tag; Hekmat M. Tantawy; Rasha M. Ghoneim | ||
| Department of Zoology1, Faculty of Sciences, Suez Canal University | ||
| Abstract | ||
| Soy Isoflavones have estrogenic activity and are widely used in human and animal diets. They have many useful activities in vitro and in vivo. However, Evidence is emerging that dietary isoflavones have an effect on immune system. The objectives of this study was to determine the effect of soy isoflavones on some cellular immunological parameters including through estimation of their effect on daily food intake, daily body weight gain, splenic and thymic weights, total and differential leukocytic count (TLC & DLC), IL-6 and histopathology of both thymus and spleen. A total of 30 ovariectomized female Albino rats were divided into three groups (10 females / group). Control group (C) received phytoestrogen-free casein-based diet, low soy phytoestrogens group (LF) received low phytoestrogens diet containing (7% soybeans) and high soy phytoestrogens (HF) group received high phytoestrogens diet containing (26% soybeans) for 30 days. The results revealed that dietary phytoestrogens didn’t alter daily food intake while reduced daily body weight gain significantly (P<0.05) in HF group than LF and control group. Splenic relative weight showed non-significant difference between groups while Thymus relative weight was significantly (P<0.05) reduced in LF and HF groups than control in a dose depending manner. Total leukocytic count was significantly (P<0.05) increased in LF and HF groups than control while DLC showed non-significant difference among groups. Inteleukin-6 was significantly (P<0.05) reduced in both treated groups than control. The histopathological studies of treated groups showed decreased white pulp (WP) area and cellularity with reduced number of lymphocytes especially in HF group with depletion in medullary area of thymus in LF group and apoptotic lymphocytes in HF group. These findings show that dietary phytoestrogens interfere with cellular mediated immunity in ovariectomized female rats, so they could alleviate autoimmune diseases manifestations. | ||
| Keywords | ||
| Phytoestrogens; TLC; DLC; IL-6; Overiectomized rats | ||
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