The Effect of Zinc Nanoparticles on Adult Rat Prostate Gland and the Possible Protective Role of Rutin: Histological and Biochemical Study | ||
| Egyptian Journal of Histology | ||
| Article 4, Volume 45, Issue 2, June 2022, Pages 372-385 PDF (5.53 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2021.65558.1438 | ||
| Authors | ||
| Heba Hashem* 1; Mariam Assad Amin2 | ||
| 1Anatomy and Embryology department, faculty of medicine, Ain shams university, Cairo, Egypt | ||
| 2Anatomy and Embryology Department, Faculty of Medicine, Ain shams university | ||
| Abstract | ||
| Introduction: Zinc nanoparticles (ZnNPs) usage is evolving in different industrial and medical applications. However, recently nanoparticles were reported for their harmful effects. Rutin is a flavonoid that presents in plants, many vegetables, and fruits. It is considered a strong antioxidant. Aim: To detect the effect of ZnNPs on the adult rat prostate gland and evaluating the possible protective effect of Rutin. Materials and Methods: Fifty adult male albino rats were divided into four groups: Group I (control), Group II (ZnNPs group) rats received ZnNPs in a dose of 100 mg/kg via oral gavage feeding needle for 28 days. Group III (Rutin group): rats received rutin in a dose of 50 mg/kg via oral gavage feeding needle for 28 days. Group IV (ZnNPs- Rutin group): rats received Zn NPs and rutin at a dose and route as groups II, III. After 28 days, rats were weighed; blood samples were collected then sacrificed. The prostate glands were dissected and processed for histological (LM and EM), immunohistochemical, and biochemical studies with morphometric analysis. Results: Light microscopic examination of Group II sections revealed degeneration of acini, vacuolation, and pyknotic nuclei of their lining cells. A highly statistically significant increase in thickness of the fibromuscular stroma (P ˂ 0.001) in Masson Trichrome sections and a highly statistically significant increase (P ˂ 0.001) in positive PCNA immunoreactions in the nuclei of epithelial cells were detected. Ultrathin sections showed vacuolations in acinar cells cytoplasm. Those findings are associated with a highly significant increase in Malondialdehyde, and Prostatic specific antigen. On the contrary, Group IV sections displayed restoration of prostatic acini arrangement with areas of hyperplasia and a statistically decrease in MDA, PSA. Conclusion: These findings evidenced that Rutin had a natural protective role against ZnNPs induced prostatic cell injury in rats. | ||
| Keywords | ||
| Oxidative stress; PCNA; prostate gland; rutin; zinc nanoparticles | ||
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