The effect of Phosphodiesterase type 5 inhibitor, Sildenafil and vitamin E on Isoprenaline induced Myocardial Infarction in male Rats. | ||
| Benha Medical Journal | ||
| Article 11, Volume 38, Issue 2, July 2021, Pages 532-547 PDF (662.58 K) | ||
| DOI: 10.21608/bmfj.2021.67015.1393 | ||
| Authors | ||
| Heba S. Youssef* 1; Marwa Hassan Muhammad2; Mona A. Said3 | ||
| 1Physiology department, Benha faculty of medicine | ||
| 2Physiology Department-Faculty of Medicine-Benha University | ||
| 3Physiology department, Faculty of Medicine, Benha University, Egypt | ||
| Abstract | ||
| Background and Aim: Despite great efforts during the last decades, acute myocardial infarction (MI) contributes to significant mortality statistics with growing yearly prevalence. The cardioprotective effect of the phosphodiesterase5-inhibitor, Sildenafil (Sil) against MI and its complications stills a matter of debate and controversy. So, we aimed to investigate the impact of Sil pretreatment on the outcome of isoprenaline (ISO)-induced infarct-like lesions and comparing it to that of vitamin E (Vit.E). Methods: 5 groups of rats were conducted; Control and ISO-injected group (75mg/kg; i.p) to induce infarct-like lesion in addition to 3 groups that were pretreated with either oral Sil (10mg/kg/day) and/or oral Vit.E (100IU/kg/day) for 3 weeks before injection of ISO. Results: the preconditioning with Sil or Vit.E significantly ameliorated ECG alterations, decreased serum levels of the cardiac enzyme CK-MB, and reduced cardiac injury score with no evident necrotic changes on histopathological examination that were observed in the ISO-induced MI. These changes were accompanied by significant increases in the cardiac SOD content and decreases in MDA content in addition to reduced immunohistochemical expression of transforming growth factor β (TGFβ) that plays an outstanding role in post-infarct remodeling. Conclusion: Sil and/or Vit.E exerted a prophylactic effect against MI and post-infarct remodeling, at least in part, through preserving the cardiac redox balance and antifibrotic effect by lowering TGFβ. | ||
| Keywords | ||
| MI; Sildenafil; TGFβ; Vit.E | ||
|
Statistics Article View: 252 PDF Download: 760 |
||
