DNA Fingerprinting of Two Oil Olive (Olea europaea L) Based on Different Molecular Markers | ||
| Journal of the Advances in Agricultural Researches | ||
| Article 1, Volume 23, Issue 2 - Serial Number 87, June 2018, Pages 194-205 PDF (608.79 K) | ||
| Document Type: Research papers | ||
| Authors | ||
| Nader Ragab Abdelsalam* 1; Ahmed Elsaid Abdallah2; Zahraa Sultan2 | ||
| 1Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, 21531 Alexandria, Egypt | ||
| 2Department of Food Science, Faculty of Agriculture (Saba Basha), Alexandria University | ||
| Abstract | ||
| Olive (Olea europaea L.) is an economically significant fruit and oil crop in the Mediterranean region where it has been cultivated since ancient times. Two virgin olive oils were obtained from two olive cultivars i.e. “Wetagen” and “Chemlali” collecting from 15 different plants localized in Marsa Matroh and Siwa Oasis. DNA was extracted and three molecular techniques i.e. RAPD, SSR and AFLP were used to made DNA fingerprinting and calculate the genetic similarity between both oil cultivars. Data showed that, genetic similarity between the both oil cultivars was 0.13% based on the eight RAPD primers, 0.07% for AFLP primers and 0.03% for SSR markers. From these data we have been able to use these markers with oil to fingerprint and differentiate olive oils in markets. The differences found in the DNA patterns can be used as a characteristic fingerprint of each oil with the certainty that these amplifications are only obtained with olive DNA and not with DNA from any other contaminant origin. | ||
| Keywords | ||
| Olive oils; RAPD; SSR; AFLP; Fingerprint | ||
| References | ||
|
Angiolillo, A., M. Mencuccini, and L. Baldoni (1999). Olive genetic diversity assessed using amplified fragment length polymorphisms. Theor. Appl. Genet., 98: 411 – 421
Bandelj D., J. Jakše and B. Javornik (2004). Assessment of genetic variability of olive varieties by microsatellite and AFLP markers. Euph., 136: 93-102
Bartolini G., G. Prevost, C. Messeri and G. Carignani (1998). Olive germplasm: cultivars and world-wide collections. FAO Seed and Plant Genetic Resources Service, Plant Production and Protection Division, Roma.
Bautista, R., M. C. Francisco and M. C. Gonzalo (2003). Genomic evidence for a repetitive nature of the RAPD polymorphisms in Olea europaea (olive-tree). Euph., 130:185-190.
Belaj A., M.C. Dominguez-García, S.C. Atienza, and N.M. Urdíroz (2012). Developing a core collection of olive (Olea europaea L.) based on molecular markers (DArTs, SSRs, SNPs) and agronomic traits. Tree Genet. Geno., 8: 365-378
Benincasa, C., A. Russo, E. Romano, M.E. Elsorady, and E. Perri (2011). Chemical and Sensory Analysis of Some Egyptian Virgin Olive Oils. J. Nutr. Food Sci., 38:120-124.
Bracci, T., M., Busconi, C. Fogher, and L. Sebastiani (2011). Molecular studies in olive (Olea europaea L.): overview on DNA markers applications and recent advances in genome analysis. P. Cell Rep., 30: 449-462.
Breton, C., C., Pinatel, F. Medail, F. Bonhommea and A. Berville (2008). Comparison between classical and Bayesian methods to investigate the history of olive cultivars using SSR-polymorphisms. Plant Sci., 175: 524-532.
Carriero, F., G. Fontanazza, F. Cellini and G. Gioio (2002). Identification of simple sequence repeats (SSRs) in olive (Olea europaea L.) Theor. and Appl. Genet.,104: 301-307.
Cipriani, G., M., Marrazzo, R. Marconi and A. Cimato (2002). Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theor. Appl. Genet., 104: 223-228.
Erre, P., I., Chessa, C. Muñoz-Diez and A. Belaj (2010). Genetic diversity and relationships between wild and cultivated olives (Olea europaea L.) in Sardinia as assessed by SSR markers. Genet. Resour. Crop Evol., 57: 41-54.
Fernando de la Torre, Rocío Bautista, Francisco M. Cánovas and M. Gonzalo Claros (2004) Isolation of DNA from olive oil and oil sediments: Application in oil fingerprinting. Food, Agric. & Envir., 2 (1): 84-89.
Gomes, S., P., Martins-Lopes, J. Lopes and H. Guedes-Pinto (2009). Assessing genetic diversity in Olea europaea L. Using ISSR and SSR Markers. Plant Mol. Biol. Rep., 27, 365-373.
I.O.C. (2013). Trade standard applying to olive oils and olive-pomace oil. COI/T.15/NC No 3/Rev. 7.
Irwin, S. V., P., Kaufusi, K. Banks, R. de la Pena and J. J. Cho (1998). Molecular characterization of taro (Colocasia esculenta) using RAPD markers. Euph., 99:183-189.
Kazan, K., J.M., Manners, D.F. Cameron (1993). Genetic relationship and variation in the Stylosanthes guianensis species complex assessed by random amplified polymorphic DNA. Geno., 36:43–49
Lashermes, P., P. Trouslot, F. Anthony, M.C. Combes and A. Charrier (1996). Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica. Euph., 87:59-64.
Lavi, V., M., Akkaya, A. Bhagwat, E. Lahav and P.B. Cregan (1994). Methodology of generation and characteristics of SSR DNA repeat in Avocado (Persea americana M.). Euph., 80: 171-177.
Lumaret R., N. Ouazzani, H. Michaud and G. Vivier (2004). Allozyme variation of oleaster populations (wild olive tree) (Olea europaea L.) in the Mediterranean Basin. Hered., 92: 343-351.
Martin, G.B. J.G.K., Williams, and S.D. Tanksley (1991). Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near isogenic lines. Proceedings of the Nati. Acad. of Sci. of the USA., 88: 2336–2340.
Martins-Lopes P., S. Gomes, E. Santos and H. Guedes-Pinto (2008). DNA markers for Portuguese olive oil fingerprinting. J. Agric. food chem., 56: 11786-11791
Matteo Busconi, Chiara Foroni, Massimiliano Corradi, Cristina Bongiorni, Federica Cattapan and Corrado Fogher (2003). DNA extraction from olive oil and its use in the identification of the production cultivar. Food Chem., 83:127–134
Michelmore, R.W., I. Paran and R.V. Kesseli (1991). Identification of markers linked to diseaseresistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the Nati. Acad. of Sci. of the USA., 88:9828-9832.
Montemurro, C., A., Pasqualone, R. Simeone, and W. Sabetta (2008). AFLP molecular markers to identify virgin olive oils from single Italian cultivars. Europ. Food Resear. Technol., 226: 1439-1444
Mousa, Y.M., D., Gerasopoulos, I. Metzidakis and A. Kiritsakis (1996). Effect of altitude on fruit and oil quality characteristics of mastoides olives. J. Sci. Food Agric., 71, 345-349.
Pafundo, P., C. Agrimonti, and E. Msestri (2007). Applicability of SCRB markers to food genomics: olive oil. J. of agric. And food chemistry. 55, 6052-6059
Perron, M. A., G. Gordon and J. Bousquet (1995). Species-specific RAPDfingerprints for the closely related Picea mariana and P. rubens Theor. and Appl. Gene. 91: 142-149.
Pillay, M., and S.T. Kenny (1996). Structure and inheritance of ribosomal DNA variants in cultivated and wild hop, Humulus lupulus L. Theor. Appl. Genet. 93:333–340.
Reiter, R.S., J.G.K. Williams, K.A., Feldmann, J.A., Rafalski, S.V., Tingey, and P.A. Scolnik (1992). Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and RAPDs. Proceedings of the Nati. Acad. of Sci. of the USA. 89: 1477–1481.
Rohlf, F.J. (2000). On the use of shape spaces to compare morphometric method. Hystrix, Italian J. Mammology, 11(1): 8-24.
Sefc, K., M., Lopes, D. Mendonça, and M.R.D. Santos (2000). Identification of microsatellite loci in olive (Olea europaea) and their characterization in Italian and Iberian olive trees. Mol. Ecol., 9: 1171-1173.
Sun, Q. Ni., Z., Litu, Z. Gao, and J. Huang (1998). Genetic relationships and diversity among Tibelian whets, common wheat and European spelt wheat revealed by RAPD markers. Euph., 99: 205 - 211.
Wilkie, S.E., P.G. Isaac, and R.J. Slater (1993). Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theo. and App. Gen., 86: 497–504.
Williames, J.K., A., Kubelik, K.J. Livak, J. A., Rafalski and S.V. Tingey (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Aci. Res., 18:6531-6535. | ||
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