Development of resistance in field strain of Aphis craccivora to the dinotefuran insecticides from the new class neonicotinoids and its effect on some enzymes content.

Mokbel, El-Sayed S.; Mohamed, Azza I.

doi:10.21608/eajbsc.2009.16306

	Development of resistance in field strain of Aphis craccivora to the dinotefuran insecticides from the new class neonicotinoids and its effect on some enzymes content.
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 6, Volume 1, Issue 1, December 2009, Pages 65-69 PDF (194.25 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2009.16306
Authors
El-Sayed S. Mokbel; Azza I. Mohamed
Central Agricultural Pesticides Laboratory, Agricultural Research Center, Egypt
Abstract
Resistance of cowpea aphid colony to the newest insecticide, dinotefuran was tested by selection pressure for 20 generations. Resistance ratio reached 148.8 fold in relation to susceptible strain. The LC₅₀ value of the parent (first generation) was 5.39 ppm with slope value 1.06 ±0.53 which mean that this strain was not homogenous to this insecticide. The value of LC₅₀ increased slowly with selection pressure, reached 22.24 ppm for the 10^th generation. Up to 15^th generation with the same selection pressure LC₅₀ value increased to reach to 66.19 ppm and reached to 230.71 ppm in case of 20^th generation. Resistance ratio values in relation to susceptible strain, increased until the 10^th generation to 14.34 fold, and fastly increasing to 42.7 fold and 148.8 fold to 15^th and 20^th generation respectively. Selection pressure produced some differences in total protein content and SDS- protein patterns. The analysis of esterase patterns obtained by the native- PAGE and by using α naphthyl acetate as a substrate which revealed a minor was measured.
Keywords
resistance; Aphis craccivora; Dinotefuran; Neonicotinoids; Enzymes

Statistics Article View: 290 PDF Download: 424