Microflora of the Petroleum Farm Tank Soil at Mosimi Depot Sagamu Ogun State Nigeria | ||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||
| Article 5, Volume 8, Issue 1, June 2016, Pages 41-46 PDF (142.91 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsg.2016.16476 | ||
| Authors | ||
| Agbaje A. B.1; Kunnuho J. B.1; Adeyemi S. A.1; Oyeyiola G. P.2 | ||
| 1Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria | ||
| 2Department of Microbiology, University of Ilorin, Ilorin, Nigeria | ||
| Abstract | ||
| Isolation and characterisation of microorganisms from petroleum contaminated soil was carried out on the soil samples collected from seven different spots at the NNPC Depot in Mosimi, Ogun State, Nigeria. The highest bacterial count of 2.6X106cfu/g was recorded at Tank 1 which was at the North-Eastern part of the Tank Farm while the least bacterial count of 5.0X105 cfu/g was recorded at Tank 3, about 30 metres away from Tank1. The fungal count ranged from 1.3 X 105 cfu/g to 1.7 X 105 cfu/g. The bacterial count ranged from 6.4 X 105 to 2.6 X 106. Bacillus subtilis had the highest occurrence (76.2%) among the bacterial isolate while Aspergillus niger had the highest occurrence among the fungal isolates.Using biochemical and morphorlogical characteristics that are based on established standards, the bacterial isolates were identified as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnuemoniae and Proteus vulgaris. The fungi isolated were Aspergillus flavus, Aspergillus niger, Rhizopus oryzaeand Penicillium chrysogenum. Aspergillus niger showed highest count and fastest growth pattern among all the isolated fungi. Using biochemical and morphorlogical characteristics that are based on established standards, the bacterial isolates were identified asBacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnuemoniae and Proteus vulgaris. The fungi isolated were Aspergillus flavus, Aspergillus niger, Rhizopus oryzaeand Penicillium chrysogenum. Aspergillus nigers howed highest count and fastest growth pattern among all the isolated fungi.This investigation provides information that could be useful in designing bioremediation protocols for environments polluted with petroleum products. | ||
| Keywords | ||
| Mosimi; Tank Farm; Bacillus subtilis; Aspergillus niger; bioremediation; Petroleum Products | ||
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