AMELORATIVE AND HEPATOPROTECTIVE EFFECTS OF FISETIN ON ACUTE MURINE TOXOPLASMOSIS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 11, Volume 51, Issue 1, April 2021, Pages 79-88 PDF (923.03 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2021.165943 | ||
| Authors | ||
| DALIA A. ELMEHY1; AMINA M. SALAMA2; NEMA A. SOLIMAN3; REEM A. ELKHOLY4; DINA M. TAHOON5; RASHA F.Department of Medical Parasitology MADY6; GHADA A. GAMEA2 | ||
| 1Departments of Medical Parasitology, | ||
| 2Departments of Medical Parasitology | ||
| 3Departments of Medical Biochemistry | ||
| 4Departments of Medical Pharmacology | ||
| 5Departments of Medical Pharmacology | ||
| 6Department of Medical Parasitology | ||
| Abstract | ||
| Toxoplasma gondii (T. gondii) protozoan invades any nucleated cell with a special predilection to reticuloendothelial cells. Liver is severely affected during acute toxoplasmosis. Promisingly, fisetin (FIS) flavonoid was proved to have antioxidant, anti-inflammatory, anti-cancerous, and anti-protozoal effects. This study was designed to evaluate the anti-Toxoplasma effect and the hepatoprotective potential of FIS in vivo. Mice were infected with the virulent RH strain of T. gondii in a dose of 1x103tachyzoites/mouse. Mice were divided into seven equal groups: G1: healthy control, G2: infected control, G3: Pyrimethamine® (PYR)/sulfadiazine (SDZ)), G4: oral-FIS, G5: oral-FIS+ PYR treated, G6: intraperitoneal (I.P) FIS, and G7: I.P FIS+PYR treated. Survival rate was estimated, and tachyzoites were counted in liver impression smears. Liver enzymes and C reactive protein (CRP) were measured in mice sera. Liver tissues were examined for inflammatory mediators; cyclooxygenase 2 (COX- 2), interleukin (IL) -10, IL-12, IL- , oxidative mediators; inducible nitric oxide synthase (iNOS), myeloperoxidase (MPO), and malondialdehyde (MDA) FIS showed a moderate effect on parasite survival and tachyzoites count, with an excellent effect in reducing serum liver enzymes activity and proinflammatory mediators. Combined (FIS+PYR) showed the best anti-parasitic and hepatoprotective actions. Its administration I.P. gave significant cure compared to oral route. FIS when given I.P than oral FIS had a good anti-inflammatory and antioxidant effect, decreased inflammatory cytokines and improved hepatic pathology of acute toxoplasmosis. Combined FIS/PYR was the best drug regimen. | ||
| Keywords | ||
| Toxoplasma gondii, Fisetin, Arginase inhibitor, Oxidative stress, iNOS; Interleukins, Liver enzymes | ||
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