ROLE OF APOPTOSIS IN EXPERIMENTAL CRYPTOSPORIDIUM PARVUM INFECTED ALBINO MICE | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 12, Volume 51, Issue 1, April 2021, Pages 89-98 PDF (1.55 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2021.165944 | ||
| Authors | ||
| REHAB M. SAMAKA1; OMAIMA K. EL SHAFEI2; NANCY M. HARBA2; SHAIMAA A. FARAG3; OSAMA F. SHARAF3 | ||
| 1Departments of Pathology | ||
| 2Departments of Medical Parasitology | ||
| 3Department of Clinical and Molecular Parasitology | ||
| Abstract | ||
| Cryptosporidium is one of the commonest opportunistic parasites. But, its chemotherapeutic options are limited, with controversy about the role of apoptosis in cryptosporidiosis. Caspases are key molecules of apoptosis. The study assessed the role of apoptosis in cryptosporidiosis by studying the relationship between the effects of phenyl vinyl sulfone (PVS), nitazoxanide (NTZ) and combined therapy on Cryptosporidium parvum infection and caspase 3 immunostaining. Ninety female laboratory bred Swiss Albino mice were divided into two major groups; immunocompetent and immunosuppressed. Both groups were divided into the following subgroups, for each one respectively: negative control (1 & 6), infected control (2 & 7), infected treated with PVS (3 & 8), infected treated with NTZ (4 & 9) and infected treated combined (5 & 10). Sacrification were done on 18th day post infection. Histopathological study of ileum was done and endogenous developmental stages were counted. Also, caspase 3 immunohistochemical staining was carried out on ileal sections. Combined therapy in groups 5 &10 showed the highest reduction in mean number of endogenous developmental stages as in G10 (P <0.05). Caspase expression in PVS treated groups (3 & 8) showed significant decrease when compared to their control groups (P < 0.05). While there were significant increase in caspase expression in groups treated with NTZ (4 & 9) when compared to their contorls (P < 0.05). Caspase 3 epithelial overexpression was evident in infected immunocompetent G2 than immunosuppressed G7 (P<0.05). | ||
| Keywords | ||
| Apoptosis; Caspase 3; Cryptosporidium; Cysteine protease inhibitors; Nitazoxanide; Phenyl vinyl sulfone | ||
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