Characterization and Identification of Moderately Thermophilic Bacteria Isolated from Jazan Hot Springs in Saudi Arabia | ||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||
| Article 7, Volume 6, Issue 1, June 2014, Pages 67-75 PDF (330.23 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsg.2014.16631 | ||
| Authors | ||
| Mohammed A. A. Sarhan; Saad Alamrri | ||
| Department of Biological Sciences, College of Science, King Khalid University, Saudi Arabia | ||
| Abstract | ||
| Three new moderately thermophilic bacteriawere isolated from three (Al-Khawbah, Bani Malik, and Al-Bozah) hot springs located in Jazan district, south-western of Saudi Arabia.The isolates were identified by the sequence analysis of the 16S rRNA gene. The 16S rRNA gene sequence alignment with GenBank resulted in a percent identity (% ID) with closest GenBank match of 98% suggesting that the isolates are new strains within the branching of well-defined genus Bacillus. The phylogenetic analysis of these strains using their 16S rDNA sequence data showed that strains SA and SS30 had highest homology (98%) with B. licheniformis, whereas strain SA09 showed 98% similarity with B. subtilis. The morphological, biochemical, and physiological characteristics of the isolates were also studied. They were aerobic, gram-positive, rod-shaped, and moderately thermophilic bacteria (with an optimum growth temperature of 45–50˚C, and pH of 6). | ||
| Keywords | ||
| Thermophilic bacteria; Bacillus; Hot spring; isolation; 16s rRNA gene | ||
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