INFLUENCE OF DIFFERENT PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND FLAVONOID CONTENTS IN ALFALFA (Medicago sativa L.).

Mohamed, M. K.; Shehab, G. G.; Mahmoud, Abeer A.; Amer, Alia A.

doi:10.21608/jpp.2008.166612

	INFLUENCE OF DIFFERENT PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND FLAVONOID CONTENTS IN ALFALFA (Medicago sativa L.).
Journal of Plant Production
Article 5, Volume 33, Issue 6, June 2008, Pages 4089-4103 PDF (827.49 K)
Document Type: Original Article
DOI: 10.21608/jpp.2008.166612
Authors
M. K. Mohamed¹; G. G. Shehab²; Abeer A. Mahmoud¹; Alia A. Amer³
¹Botany Department, Plant Physiology Section, Faculty of Agriculture, Cairo University, Giza, Egypt.
²Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
³Plant Biotechnology Research Lab, Faculty of Agriculture, Cairo University, Giza, Egypt.
Abstract
Alfalfa contains many important substances including flavonoids which play many diverse roles in plant. Alfalfa callus was induced on media containing 6- benzyl aminopurin (BA), α -Naphthalene acetic acid (NAA), 2, 4 Dichloro phenoxy acetic acid (2, 4-D) and kinetin. The best callus formation was obtained from the medium of 2 mgl^-1 2, 4- D + 1.5 mgl^-1 Kinetin; leaf was the best organ of alfalfa in production of fresh weight. The 2^nd subculture was higher in growth characters and flavonoids production as compared to the 1^st subculture. Flavonoid concentration was higher in shoot tip of 1^st subculture and leaves in the 2^nd subculture.
Keywords
Alfalfa; callus; plant growth regulators; flavonoid

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