Molecular study of Beta-Haemolytic Streptococci in Patients with Tonsillitis In Ramadi City | ||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||
| Article 6, Volume 3, Issue 1, December 2011, Pages 33-39 PDF (999.34 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsg.2011.16693 | ||
| Authors | ||
| Samira M. Al-Khalifawi1; Alaa A. Al-Khafaji2; Muthana A. Al-zobaei2 | ||
| 1Medical Microbiology, Al-Ramadi Hospital | ||
| 2Medical Microbiology, Microbiol. Dept., College of Medicine, University of Anbar | ||
| Abstract | ||
| Objectives: To evaluate the sensitivity and specificity of polymerase chain reaction PCR technique in early detection of infection with group A beta-haemplytic streptococci in comparing with conventional diagnostic methods among the patients with tonsillitis. Methods: One hundred ninety one swabs and blood specimens were obtained from patients with tonsillitis in addition to samples of healthy control group during a period between 2008-2009 in general Ramadi hospital, Iraq and the tests (PCR) and other bacteriological and serological test at the same time on these samples. Results: Our data show that in recurrent tonsillitis group out of 72 GBHS isolates 43 (59.7%) were detected by PCR, however 15(20.8%) were positive by serogrouping. Out of 75 bacterial isolates, 22 (29.4%) isolates were GABHS and 15 (83.5%) of these were GABHS detected by PCR and only 4 (5.3%) of GABHS detected by serogrouping methods in acute recurrent group, and our results revealed that the inoculation of penicillin and L-therionine to the brain heart infusion broth improved the DNA extraction from gram positive bacteria (GBHS). Conclusion: Our study confirmed that the PCR technique more sensitive and highly specific in early detection of GBHS among the patients with tonsillitis in comparing with conventional; diagnostic methods. | ||
| Keywords | ||
| type A Streptococcus; PCR; Tonsillitis | ||
|
Statistics Article View: 268 PDF Download: 401 |
||
