Determination ofTaGSK1 gene expression in selected wheat under different salt stresses | ||
| Egyptian Academic Journal of Biological Sciences, H. Botany | ||
| Article 8, Volume 5, Issue 1, December 2014, Pages 85-92 PDF (471.48 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsh.2014.16832 | ||
| Authors | ||
| Eman Noaman Ismail1; Norrya A. Ali2; Majeed Arsheed Sabbah1 | ||
| 1Biotechnology research center, Alnahrain University, Baghdad, Iraq | ||
| 2Institute of genetic engineering and biotechnology, Baghdad University | ||
| Abstract | ||
| TaGSK1 gene was shown to be differentially expressed in salt tolerant wheat plants. This study was conducted to reveal the expression of this gene in four selected wheat cultivated in Iraq. Two wheat cultivars (Dijila and Furat) and one genotype (2H) were grown in three salt levels, 0, 15, 25 ds/m. Meanwhile, cultivar (Tamooze 2) was grown only at zero level salt. Total RNA of the four plants was isolated, cDNA was synthesyzed and PCR amplification was tried. The results showed that a fragment of 189 bp was amplified in the selected salt tolerant wheat but not amplified in control one. In addition, TaGSK1 gene was induced in salt tolerant wheat only and its expression level increased with increasing salt concentration as determined by SYBR green real time PCR. These results indicated the suitablility of TaGSK1 gene as salt-tolerance marker in wheat under study. | ||
| Keywords | ||
| PCR; plant; salt, TaGSK1; Wheat | ||
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