El-Sabrout, A., Zaharan, H. (2016). Triflumuron Effects as Insect Growth Inhibitor (IGI) on Amino-Acids Contents on Adults Reproductive Tracts of Spodoptera littoralis (Boisd.). test, 22(1), 148-163.
Ahmed Mohmed El-Sabrout; Hossam Eldin Magdy Zaharan. "Triflumuron Effects as Insect Growth Inhibitor (IGI) on Amino-Acids Contents on Adults Reproductive Tracts of Spodoptera littoralis (Boisd.)". test, 22, 1, 2016, 148-163.
El-Sabrout, A., Zaharan, H. (2016). 'Triflumuron Effects as Insect Growth Inhibitor (IGI) on Amino-Acids Contents on Adults Reproductive Tracts of Spodoptera littoralis (Boisd.)', test, 22(1), pp. 148-163.
El-Sabrout, A., Zaharan, H. Triflumuron Effects as Insect Growth Inhibitor (IGI) on Amino-Acids Contents on Adults Reproductive Tracts of Spodoptera littoralis (Boisd.). test, 2016; 22(1): 148-163.

Triflumuron Effects as Insect Growth Inhibitor (IGI) on Amino-Acids Contents on Adults Reproductive Tracts of Spodoptera littoralis (Boisd.)

Journal of the Advances in Agricultural Researches
Article 10, Volume 22, Issue 1 - Serial Number 82, March 2017, Pages 148-163    PDF (1.44 M)
Document Type: Research papers
Authors
Ahmed Mohmed El-Sabrout* ; Hossam Eldin Magdy Zaharan
Applied Entomology Department, Faculty of Agriculture (El-Shatby), University of Alexandria, Egypt.
Abstract
The present study was designed to evaluate the physiological-insecticidal activity of triflumuron as IGI on amino acids content in the testes and ovaries of both sexes reproductive tracts of Spodoptera littoralis. The lethal dose 50% of those individuals subjected to triflumuron (LD50=0.006 μl/larva) was topically applied on newly molted fifth instar larvae of the cotton leafworm S. littoralis. The treatment decreased the amounts of amino acids content of testes of adult males (2 days-old) obtained from treated larvae. This content was highly noticed and were ranged between 0.00 to 9.65% in 9 amino acids from 16 total amino acids examined i.e.  DL-2-Amino-N-butyric Acid, Tryptophan(TRP), Isoleucine(ILE), DL-Threonine(THR), L-Arginine(ARG), L- Leucine(LEU), L- Alanine(ALA), DL- Serine(SER) and L- Lysine(LYS), while these amino acids amount percentages were ranged between 3.45 to 16.94%. The present results showed strong effects of LD50 of triflumuron on the major amino acids that may stimulate and activate certain physiological functions in spermatogenesis in the testes of male reproductive tract i.e. SER and LYS. These latter amino acids in control males (2days-old) were 5.51 and 3.45%, respectively, but they were zero% in treated males.  Both amino acids SER and LYS may play many important roles in the structure of both types of spermatozoa of the cotton leafworm. Moreover, the greatest decrease in amino acid content was observed especially in case of LYS (zero %) with triflumuron (LD50) , while it was 19.92% in control female. LYS may play a specific role in the structure of egg of the cotton leafworm. The greatest increase of amino acid content was noticed in case of ASPARTIC ACID (ASP) (17.40%) with triflumuron (LD50), while it was zero% in control female. The average weight of ovaries andtesticles were recorded (32.21, 1.08 mg) with treated female and male by triflumuron in respect, while it was 78.65, 3.74 mg in control female and male, respectively. The results indicated that the biochemical composition of both sexes reproductive tracts were reduced. Triflumuron caused reproductive suppression, also, it affects the amino acids amount and it had its function on adults reproductive tracts, ultimately leading to reduce sperm transfer from the treated male insect to female.
Keywords
Triflumuron; Spodoptera littoralis; Amino acids; reproductive tract and physiological functions
References
Abo-El-Ghar, M.R., M.E. Nassar, M.R. Riskalla and S.F. Abd-El-Ghafar (1986). Rate of development of resistance and pattern of cross-resistance in fenvalerate and decamethrin resistant strains of Spodoptera littoralis. Agric. Res., 61: 141-145.

Deyl,  Z., J. Hyanek,  and M.Horokva (1986). Profiling of amino acids in body fluids and tissues by means of liquid chromatography. J Chromatogr., 379: 177-250.

Dinan, L. (1989). From chemistry to mode of action. Ed. By Koolman JS: Thieme, pp. 345-354.

El-Sabrout, A. (2013). Effects of some materials from plant origin on the cotton leafworm, Spodoptera littoralis. Ph.D. Thesis, Fac. of Agriculture, Alexandria Univ., Egypt.

El-Zoghby, Fadia (1980). Studies on the effects of some materials from plant origin on insects. Ph.D. Thesis. Faculty of Agric. Univ. of Alexandria, Egypt.

Engelmann, F. (1979). Insect vitellogenin: identification, biosynthesis, and role in vetillogenesis. Adv. Insect Physiol., 27: 49-108.

Friedländer, M. (1997). Control of the Eupyrene-Apyrene sperm Dimorphism in Lepidoptera. J. Insect Physiol. 43(12):1085-1092.

Friedländer, M. and S.E. Reynolds (1988). 20-Hydroxyecdysone unblocks meiotic metaphases during spermatogenesis of the tobacco hornworm, Manduca sexta. J. Insect Physiol., 34: 1013-1019.

Gäde, G., K.H. Hoffman and J.H. Spring (1997). Hormonal regulation in insets: Facts, Gaps, and future directions, Physiol. Rev., 77(4): 963-1032.

Indrasith, L. S., T. Sasaki, T. Yaginuma and O. Yamashita (1988). The occurrence of a premature form of egg-specific protein in vitellogenic follicles of Bombyx mori. J. Comp. Physiol., 158: 1-7.

Kanost, M. R., J. K. Kawooya, J. H. Law, R. O. Ryan, M. C. Van Heusden, and R. Ziegler (1990). Insects haemolymphproteins, Adv. Insect Physiol.,  22: 299-396.

Khebbeb, M. E. H., R. Gaouaoui and F. Bendjeddou (2008). Tebufenozide effects on the reproductive potentials of the mediterranean flour moth, Ephestia kuehniella African.  J. Biotechnol., 7 (8): 1166-1170.

Mancini, K. and H. Dolder (2004). Protein detection in spermatids and spermatozoa of the butterfly Euptoieta hegesia (Lepidoptera).  BIOCELL 21(1): 13-23.

Nation, J. L. (2001). Insect Physiology and Biochemistry, Reproduction, chapter 15:425-445.

Perveen, F. and T. Miyata (2000). Effects of sublethal dose of chlorfluazuron on ovarian development and oogenesis in the common cutworm Spodoptera litura (Lepidoptera: Noctuidae). Ann. Entomol. Soci. Amer., 93 (5): 1131-1137.

Seth, R.K., J.J. Kaur, D.K. Rao and S.E. Reynolds (2004). Effects of larval exposure to sublethal concentrations of the ecdysteroid agonists RH-5849 and tebufenozide (RH-5992) on male reproductive physiology in Spodoptera litura. J. Insect Physiol., 50: 505–517.

Sobeiha, A. K. , H.A. Sallam and S. S.A. El-Shall (2000). Bio-6 Amino Acid Content of the Gamma Irradiated Cotton Leaf-Worm, Spodoptera littoralis (Boisd.) 7th Conf. Nuclear Sci. & Applications, 6-10 February 2000. Cairo, Egypt.

Soltani, N. (1990). Action du diflubenzuron et de la 20-hydroxyecdusone sur les glucides et les proteines hemolymphtiques chez les nymphes de Tenebrio molitor L.(Coleoptera: Tenebrionidae). Ann. Soc. Ent. Fr., 26: 575-584.

Soltani, N. and N. S. Mazouni  (1992). Diflubenzuron and oogenesis in the codling moth, Cydia pomonella (L.). Pestic. Sci., 34: 257-261.

Soltani, N., N. Soltani, B. Mauchamp and J. P. Delbeque (1989). Effects of diflubenzuron on the ecdysteroid titres in two insect species. Tag.-Ber. Akad. Landwirtech. Wiss. 274: 171-177.

Sun, X., B.A. Barrett and D.J. Biddinger (2000). Fecundity and fertility reductions in adult leafrollers exposed to surfaces treated with the ecdysteroid agonists tebufenozide and methoxyfenozide. Entomol. Exper. Applic., 94: 75–83.

Wedell, N. (2005). Female receptivity in butterflies and moths. J. Experim. Bio., 208: 3433-3440.

Wing, K.D. (1988). RH-5849, a non steroidal ecdysone agonist: effects on a Drosophila cell line. Sci., 241: 464-469.

Wright, J. E., and G. E. Spates (1976). Reproductive inhibition activity of the insect growth regulator Th-6040 against the stable and house fly: effect on hatchability. J. Econ. Entomol., 69: 365-368.

Statistics
Article View: 78
PDF Download: 235