PRIMITIVE EFFECTS OF CULTURE SYSTEM, SORBITOL AND SALICYLIC ACID ON GROWTH OF TISSUE CULTURE DERIVED DATE PALM PLANTS (PHOENIX DACTYLIFERA L.) AMRI CV. DURING ACCLIMATIZATION STAGE | ||
| Menoufia Journal of Plant Production | ||
| Article 3, Volume 6, Issue 4, April 2021, Pages 221-233 PDF (689.06 K) | ||
| Document Type: original papers | ||
| DOI: 10.21608/mjppf.2021.169831 | ||
| Authors | ||
| M. R.M. Rabeh1; E.G. Gadalla2; S.A. Ahmed1; M.S. Shehata2 | ||
| 1Horticulture (Pomology) Department, Fac. of Agric., Menoufia Univ. | ||
| 2The Central Laboratory for Date Palm Researches and Development, Agricultural Research Center, Giza, Egypt. | ||
| Abstract | ||
| Date palm plantlets produced in vitro using tissue culture technique in a greenhouse face a great challenge that may lead to their death due to different ambient environmental conditions, moreover the slow growth rate of the survived tissue culture derived date palm plants during acclimatization are critical problems facing the tissue culture as a commercial technique of date palm propagation. Therefore, the success of date palm micropropagation is strongly linked to the success and quality of plantlets in the greenhouse. This study aimed to evaluate the effect of three different cultural systems on survival percentage, plant length, number of leaves and estimation photosynthetic pigments in greenhouse in the first experience as well as effect of foliar spray and watered three concentrations of Sorbitol (0, 15, 30 and 45 g l-1) and salicylic acid at concentrations (0, 20, 40 and 60 mg l-1) on some vegetative growth parameters such as survival percentage, leaf width and growth vigor in the secondary experience. Findings indicated that using both the hydroponic (first culture system) and the traditional systems, with the addition of Barbary-Plante (third culture system) during the acclimatization stage gave highest values of survival percentage, plant length, number of leaves and chlorophyll a+b content compared to the second culture system, where the lowest values were recorded. Also spraying and irrigation with sorbitol at 30 gl-1 plus salicylic acid at 40 mg l-1 giving the highest values of survival percentage, leaf width and growth vigor (91%, 2.73 cm, 4, respectively) compared to other treatments. | ||
| Keywords | ||
| Microprpagation; Date palm; Acclimatization stage; System culture - Salicylic acid; Sorbitol | ||
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