HPLC analysis of non-enzymatic antioxidants in Azolla caroliniana (Pteridopsida) subjected to UV-B | ||
| Egyptian Academic Journal of Biological Sciences, H. Botany | ||
| Article 3, Volume 3, Issue 1, December 2012, Pages 19-30 PDF (932.59 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsh.2012.17003 | ||
| Authors | ||
| E. M. Mostafa; M. M. Ibrahim | ||
| Botany and Microbiology Department, Faculty of Science, Alexandria University, 21511, Alexandria, Egypt | ||
| Abstract | ||
| The ultrastructure of chloroplast and quantified antioxidant compounds such as α-tocopherol, ascorbic acid and beta carotene, semi quantitatively by comparing the peak areas of HPLC chromatograms, were affected considerably in response to exposure of Azolla caroliniana to UV-B radiation for 24 and 48 h. The results show that the most striking effect of the enhanced UV-B treatment on the ultrastructure of A. caroliniana was observed after 48 h as markedly widened chloroplast area occupied by large amount of plastoglobuli in stressed fronds. Prolonged UV-B exposure of A. caroliniana for 48 h resulted in a reduction in peaks area for for α-tocopherol, ascorbic acid and beta-carotene. The results show that changes in the chromatogram of non-enzymatic antioxidants were correlated with the duration of exposure to UV-B radiation. These antioxidant metabolites play a vital role in protecting A. caroliniana to a certain limit against UV-B radiation. | ||
| Keywords | ||
| HPLC- antioxidants- Azolla caroliniana- UV-B | ||
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