NEW AZOTOBACTER TRANSFORMANTS TO IMPROVE GROWTH OF SQUASH PLANTS UNDER SALINITY STREESS. | ||
| Journal of Plant Production | ||
| Article 16, Volume 33, Issue 8, August 2008, Pages 5963-5973 PDF (519.08 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2008.170398 | ||
| Authors | ||
| E. A. Hassan1; O. F. Dakhly2 | ||
| 1Central Lab. of Organic Agriculture | ||
| 2Genetic Dept., Fac.Agric. Minia Univ. | ||
| Abstract | ||
| This study was carried out at the laboratory of genetic department and the farm of horticulture department Fac. of Agri. Minia University in two successive seasons on f 2003 and 2004. In this study some transformants of Azotobacter showed high tolerance against saline water (0.25, 0.50, 0.75, 1.oo, 1.25, 1.50, 1.75, and 2.00 g/ 100 ml w/v). Azotobacter transformants were obtained by incubating wild isolate of Azotobacter with an isolate of Bacillus spp., tolerant to 30% NaCl, for 2 or 24 hrs.These resulted transformants were tested in pot experiments under four levels of salinity and presence of squash plants as indicator. The results revealed that: 1- The wild type strain of Azotobacter was sensitive to 1.5-2.0% NaCl as recipient comparison to the Bacillus spp < /em>.which resistant to 30%NaCl as donor. 2- The transformants of Azotobacter were obtained after two hours incubation were less in their frequencies than those were obtained after 24 hours incubation with DNA of Bacillus sp. 3- Adding transformants of Azotobacter to salinity soil led to reduction in the symptoms of salinity on squash plants. 4- Different transformants varied in their effects, regards, plant growth characters under salinity conditions and T2 & T4 showed the best effect. | ||
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