Light and Electron Microscopic Study on the Possible Protective Effect of Pomegranate Peel Extract on the Pituitary- Thyroid Axis Exposed to Monosodium Slutamate in Adult Male Albino Rats | ||
| Egyptian Journal of Histology | ||
| Article 2, Volume 45, Issue 3, September 2022, Pages 667-686 PDF (9.66 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2021.68994.1449 | ||
| Authors | ||
| dalia abd elrazik noya* 1; mona abd elmawla soliman2; manar Aly bashandi3 | ||
| 1histology and cell biology department faculty of medicine menoufia university | ||
| 2Histology&Cell biology department, faculty of medicine, menoufia university | ||
| 3department of anatomy and emberyology, faculty of medicine, menoufia university ,shebin el kom ,egypt | ||
| Abstract | ||
| Introduction: Monosodium glutamate is one of our new high-tech food additives. Nowadays, use of natural antioxidant as pomegranate peel extract has become an attractive therapeutic strategy for reducing the risk of disease. Aim of Work: This study was designed to assess the effects of MSG on the pituitary thyroid axis and the possible protective role of pomegranate peel extract. Material and Methods: Forty adult male albino rats were divided into three groups. Group I: (control) that was subdivided into two subgroups: I A, and I B. Group II (MSG treated) received MSG at a dose of (6mg/g./day). Group III (MSG & Pomegranate peel treated) received PPE &MSG as same dose as subgroup I B and group II. At the end of the experiment, pituitary and thyroid glands were dissected out and processed for biochemical, histological, immune-histochemical, and electron microscopic studies. Results: MSG caused thyroid follicles distortion and degeneration. Follicular cells possessed vacuolated cytoplasm, and pyknotic nuclei. Decreased colloids & inflammatory infiltrates were detected. By E/M, follicular cells showed dilated RER cisternae, degenerated mitochondria, & depleted microvilli. While, basophils of pituitary gland appeared degenerated with vacuolated cytoplasm. By E/M, thyrotrophs showed dilated RER cisternae, dilated Golgi apparatus, and degenerated mitochondria. There was a highly significant decrease (P value˂0.001) in the mean serum level of T3, T4 and TSH in Group II as compared with group I. While group III showed a highly significant increase (P value˂0.001) in the same parameter as compared with group II. The administration of PPE induced improvements of these changes. Conclusion: This work demonstrated that pomegranate peel extract improves the histological & biochemical changes induced by MSG on the pituitary thyroid axis. | ||
| Keywords | ||
| Monosodium glutamate; pituitary thyroid axis; pomegranate peel extract | ||
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