Attallah, A., Mansy, S., Zarouk, W., Mohamed, I. (2010). Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer. test, 40(1), 306-313. doi: 10.21608/ejhm.2010.17380
Abdelfattah M. Attallah; Seham E. Mansy; Waheba A. Zarouk; Ibrahim E. Mohamed. "Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer". test, 40, 1, 2010, 306-313. doi: 10.21608/ejhm.2010.17380
Attallah, A., Mansy, S., Zarouk, W., Mohamed, I. (2010). 'Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer', test, 40(1), pp. 306-313. doi: 10.21608/ejhm.2010.17380
Attallah, A., Mansy, S., Zarouk, W., Mohamed, I. Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer. test, 2010; 40(1): 306-313. doi: 10.21608/ejhm.2010.17380

Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer

The Egyptian Journal of Hospital Medicine
Article 2, Volume 40, Issue 1, July 2010, Pages 306-313    PDF (775.75 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2010.17380
Authors
Abdelfattah M. Attallah1; Seham E. Mansy2; Waheba A. Zarouk3; Ibrahim E. Mohamed4
1R & D Department, Biotechnology Research Center, New Damietta City, Egypt
2Faculty of Science, Mansoura University, Damietta Branch, Egypt
3Molecular genetics and enzymology department, National Research Center,Cairo, Egypt
4Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Abstract
Background: the highest incidence rates of bladder cancer are generally found in industrially developed countries, particularly North America and
Western Europe, and areas associated with endemic schistosomiasis, including parts of Africa and the Middle East . The appropriate treatment of patients with bladder cancer mandates early detection and regular follow up for recurrences . Currently, cystoscopy is the standard method for diagnosing and monitoring bladder cancer recurrence, but it is an invasive and relatively costly technique, and may sometimes be inconclusive, particularly in cases of cystitis .
Patients and methods: western blot and specific immunoglobulin-G antibody were used to identify the urinary NMP marker. Urine samples from 123 patients
with bladder cancer and 50 controls were evaluated using the developed SDS-PAGE, Western blot and ELISA.
Results: the NMP marker was identified in the urine of
patients with bladder cancer at 52 kDa (NMP-
52) by SDS-PAGE, Western blot and ELISA. In addition, the NMP-52 tumor marker was not detected in the urine
of patients .
Conclusion: detecting the urinary NMP-52 marker using SDS-PAGE, Western blot and ELISA, would be helpful in the rapid diagnosis of bladder cancer.
 
Keywords
bladder; Tumor; marker; NMP; urine; Diagnosis; SDS - PAGE; western blot; ELISA
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