Hassan, F., Basyoni, H., Abdelfattah, D., Abdel Razik, H. (2021). MESENCHYMAL STEM CELLS RIF1, TCL1A, AND TERT MARKERS AS INDICATORS OF AGE ESTIMATION IN RATS. test, 21(2), 33-45. doi: 10.21608/ejfsat.2020.27855.1140
Fatma Mohamed Hassan; Hoda Ahmed Mohamed Basyoni; Dina Sabry Abdelfattah; Heba Abdo Abdel Razik. "MESENCHYMAL STEM CELLS RIF1, TCL1A, AND TERT MARKERS AS INDICATORS OF AGE ESTIMATION IN RATS". test, 21, 2, 2021, 33-45. doi: 10.21608/ejfsat.2020.27855.1140
Hassan, F., Basyoni, H., Abdelfattah, D., Abdel Razik, H. (2021). 'MESENCHYMAL STEM CELLS RIF1, TCL1A, AND TERT MARKERS AS INDICATORS OF AGE ESTIMATION IN RATS', test, 21(2), pp. 33-45. doi: 10.21608/ejfsat.2020.27855.1140
Hassan, F., Basyoni, H., Abdelfattah, D., Abdel Razik, H. MESENCHYMAL STEM CELLS RIF1, TCL1A, AND TERT MARKERS AS INDICATORS OF AGE ESTIMATION IN RATS. test, 2021; 21(2): 33-45. doi: 10.21608/ejfsat.2020.27855.1140

MESENCHYMAL STEM CELLS RIF1, TCL1A, AND TERT MARKERS AS INDICATORS OF AGE ESTIMATION IN RATS

The Egyptian Journal of Forensic Sciences and Applied Toxicology
Article 4, Volume 21, Issue 2, June 2021, Pages 33-45    PDF (574.52 K)
Document Type: Original Article
DOI: 10.21608/ejfsat.2020.27855.1140
Authors
Fatma Mohamed Hassan* 1; Hoda Ahmed Mohamed Basyoni2; Dina Sabry Abdelfattah3; Heba Abdo Abdel Razik2
1Forensic medicine and clinical Toxicology department, faculty of medicine, Cairo university
2Forensic medicine and Clinical toxicology, Faculty of medicine, Cairo University, Egypt.
3Medical Biochemistry and Molecular Biology, Faculty of medicine, Cairo University, Egypt
Abstract
Background: Research on stem cells has become one of the most promising and advanced scientific topics. Nevertheless, there is a lack of this type of research in the field of forensic diagnostics. Age estimation is one of the main worked-on issues in forensic practice and research. Still, there is a need to explore new complementary reliable methods. This study aimed to detect the possibility of age estimation via age-associated alteration of RIF-1 (Replication timing regulatory factor 1), TCL1A (T-cell leukemia/lymphoma-1A), and TERT (Telomerase reverse transcriptase) gene expression in rats bone marrow stem cells. Methods: Isolation of bone marrow mesenchymal stem cells from rats were undertaken at different ages; 2, 4, 6, 8, 10, and 12 weeks old. RNA extraction was performed then total RNA was reverse transcribed to cDNA to detect the quantitative expression of RIF-1, TLC1A, and TERT by using the qRT-PCR then immunoblotting. Results: There were statistically significant increases in RIF1 and TCL1A mRNA and protein expression and a decrease in TERT mRNA and protein expression with age. Conclusion: Our results showed that the mRNA and protein expression of RIF-1, TCL1A, and TERT in bone marrow cells of rats could be useful indicators for age estimation in forensic practice.
Keywords
age estimation; mRNA; RIF-1; TCL1A; TERT; mesenchymal stem cells
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