Olowe, B., Arogbodo, J. (2022). Molecular identification of virulence and antibiotic-resistant gene in Escherichia coli O157 and non-O157 recovered from water samples. test, 3(2), 348-359. doi: 10.21608/mid.2021.74664.1148
Busayo Olowe; Joseph Arogbodo. "Molecular identification of virulence and antibiotic-resistant gene in Escherichia coli O157 and non-O157 recovered from water samples". test, 3, 2, 2022, 348-359. doi: 10.21608/mid.2021.74664.1148
Olowe, B., Arogbodo, J. (2022). 'Molecular identification of virulence and antibiotic-resistant gene in Escherichia coli O157 and non-O157 recovered from water samples', test, 3(2), pp. 348-359. doi: 10.21608/mid.2021.74664.1148
Olowe, B., Arogbodo, J. Molecular identification of virulence and antibiotic-resistant gene in Escherichia coli O157 and non-O157 recovered from water samples. test, 2022; 3(2): 348-359. doi: 10.21608/mid.2021.74664.1148

Molecular identification of virulence and antibiotic-resistant gene in Escherichia coli O157 and non-O157 recovered from water samples

Microbes and Infectious Diseases
Article 14, Volume 3, Issue 2, May 2022, Pages 348-359    PDF (764.71 K)
Document Type: Original Article
DOI: 10.21608/mid.2021.74664.1148
Authors
Busayo Olowe1; Joseph Arogbodo* 2
1Department of Biological Sciences, Faculty of Basic Medicals and Applied Science, Lead City University, Ibadan, Oyo State, Nigeria
2Department of Animal Production and Health, Federal University of Technology Akure, Ondo State, Nigeria
Abstract
Background:  This research work focused on ascertaining the presence of virulence and antibiotic-resistant genes in Escherichia coli (E. coli) O157 and non-O157 recovered from drinking water sources. Methods: Identification of E. coli O157 and non-O157 was carried out using standard serological and PCR techniques. Virulence genes (rfb O157, fliC H7, stx1, stx2, eae and hly genes) and antibiotic-resistant gene (BlaTEM) were detected using PCR method on selected isolates (n= 15) from different water sources which demonstrated multiple antibiotic-resistance in a previous study. Results: The serological identification result revealed that a total of 68 out of 382 E. coli isolates, recovered in a previous work, were identified as a presumptive E. coli O157. These included 19.1 %, 21.7 %, 33.3 %, 14.3 % and 9.1 % of E. coli isolates from wells, boreholes, sachets, streams and pipe-borne respectively. Statistical analysis revealed that there was no significant difference in the frequency of E. coli O157 from the different water sources (p > 0.05). Also, there was a statistically significant positive correlation between the E. coli isolates and E. coli O157 (Pearson’s r = 0.996). Detection of virulence and antibiotic-resistant genes showed that only 46.7 %, 33.3 %, 33.3 %, 93.3 %, and 66.7 %  carried  rfb O157,  fliC H7, stx1, stx2 and rpoS gene respectively. In contrast, all the isolates possessed hly and BlaTEM genes but none had eae gene. Conclusion: The presence of one or combination of these genes in these isolates depicts their virulence and resistance nature.
Keywords
Escherichia coli O157:H7; non-O157; virulence genes; pathogenic genes
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