IMPROVEMENT OF RABBIT PASTEURELLOSIS VACCINE USING MONTANIDE ISA50 | ||
| Egyptian Journal of Agricultural Research | ||
| Article 22, Volume 89, Issue 2, July 2011, Pages 697-708 PDF (253.35 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2011.176060 | ||
| Authors | ||
| ELHAM A. YOUSSEF; HODA E. TAWFIK | ||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt. | ||
| Abstract | ||
| Montanide ISA50 was used as an adjuvant for preparation of inactivated rabbit pasteurellosis vaccine, and compared with the commercially produced oil inactivated rabbit pasteurellosis vaccine adjuvanted with mineral oil. Two groups of rabbits were inoculated separately by first dose of these vaccines followed by second dose after 21 days from the first dose, the mineral oil used for first group and ISA50 used for the second group as adjuvant, meanwhile, the third group was left as non-vaccinated control group. Cellular immunity was evaluated by using cell proliferation test and macrophage activity test (phagocytic percentage, phagocytic index). Humoral immune response was evaluated by using indirect haemagglutination test (IHA) and enzyme linked immunosorbent assay (ELISA). The results of the aforementioned tests proved that the two types of adjuvant induced protective titre against the diseases, but Montanide ISA50 oil adjuvant gave higher and longer duration of antibody level than the mineral oil. Challenge test and protection test in rabbit and mice gave similar results. | ||
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