Expression of CD38 and CXCR3 in children with atopic dermatitis. | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 1, Volume 33, Issue 1, October 2008, Pages 467-478 PDF (379.75 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejhm.2008.17610 | ||
| Authors | ||
| Fawzia A. EL.Sheshtawy; Naglaa A . Ahmad; Manal Abdul Salam | ||
| Departments of Clinical Pathology, Dermatology , Pediatric # , Faculty of Medicine for Girls- Al Azhar University | ||
| Abstract | ||
| Background : Atopic dermatitis ( AD) is a chronic or chronically relapsing inflammatory skin disease with a prevalence ranging from 10% to 20% in children of developed countries. Skin-infiltrating leukocytes play a pivotal role in the initiation and amplification of atopic skin inflammation. The cytokines produced by T helper- 2 ( Th2) cells are crucial factors in the induction and maintenance of the disease . Aim: to study the percentage of expression and mean fluorescence intensity( MFI) of the activation marker CD38 and the chemokine receptor CXCR3 on peripheral blood CD3+ lymphocytes in children with atopic dermatitis . Also total serum IgE and absolute differential count were evaluated .This might be targets for therapy in disease . Methods: This study was conducted on thirty cases of AD children. Their age range was 3- 10 years. Also non atopic fifteen children age and sex matched with disease group were included as a control group. The percentage of expression of the CD38 , CXCR3 and MFI were analyzed by flow- cytometry on peripheral blood CD3+ T lymphocytes . Also total serum IgE levels was measured by immunonephelometry . The absolute eosinophil , absolute lymphocytes, absolute neutrophil count were evaluated . Results: The mean percentage of CD38 expression on CD3 + lymphocytes and MFI were 70.5% and 5.8 respectively in AD children compared with 17.8 % and 5.1 in non –atopic children healthy control ( p < 0.01 and p > 0.05 respectively ) . The mean percentage of CXCR3 expression on CD3+ T lymphocytes and MFI in AD children were 17.9 % and 2.9 respectively compared with 67.93 %and 3.3 in healthy controls (p < 0.01 and p > 0.05 respectively ). The mean of the total serum IgE in the patient group was 199.3 IU/ml compared with 62.27 IU/ml in non-atopic children (p < 0.01). Conclusion : These results suggest that there is a relation between atopic conditions and an increase in peripheral blood T lymphocyte expressing CD38% and decrease expression of CXCR3%.The presence of high expression of CD38 in atopic patients seems to confirm the role of this molecule as an activation marker useful for evaluation of Th2 immune response . whereas CXCR3-expression on CD3+ lymphocytes decreased in AD than normal control as the chemokine receptor profile determine the migratory patterns of leukocytes . These results may suggest the dysbalance between Th1/ Th2 in AD patients . | ||
| Keywords | ||
| CD 38; CXCR3; Flow- Cytometry; AD | ||
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