COMPARATIVE STUDIES BETWEEN INDIRECT HEMAGGLUTINATION (IHA) TEST AND ENZYME LINKED IMMUNOSORBENT ASSAYS (ELISA) TEST FOR DETECTION OF SERUM ANTIBODY LEVELS AGAINST INFECTIOUS BURSAL DISEASE (IBD) VIRUS IN COMMERCIAL BROILERS | ||
| Assiut Veterinary Medical Journal | ||
| Article 27, Volume 57, Issue 131 - Serial Number 4, October 2011, Pages 1-12 PDF (574.27 K) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2011.176945 | ||
| Authors | ||
| AL-H.M. DAHSHAN1; A.S. HUSSIEN2 | ||
| 1Dept. of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University | ||
| 2Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University | ||
| Abstract | ||
| This study was done to compare between IHA test and ELISA test as a serological test for detection of serum antibody levels against Infectious Bursal Disease Virus (IBDV) in Commercial Broilers, 300 Serum samples were collected from different commercial broiler farms and slaughter Shops from in and around Beni-suef Governorate. These samples were divided into three groups according to the age of the birds i.e. 0-3 weeks (100 samples), 3-6 weeks (100samples) and 6-8 weeks (100 samples).ELISA and Indirect Hemagglutination (IHA) test were used to titrate the serum antibodies against infectious bursal disease virus. Hyper immune serum was raised in laboratory rabbits against IBDV vaccine (D-78). It was used to identify and confirm the IBD virus from bursal tissues isolated from the IBD affected flocks using the qualitative tests i.e. agar gel precipitation suspension was prepared and used to determine the antibody titer. Results indicated that out of 300 test serum samples 195 (65%) were positive. The maximum positive samples belonged to 6-8 weeks of age (89%) followed by 3-6 weeks (73%) and 0-3 weeks (33%), respectively while the result of ELISA test of high and low titers were 18% and 82 % at 0-3 week of age then at age 3-6 weeks the high titers were 35% and low titers were 65% and finally high ELISA titers 69% and low titers 31%at age 6-8 weeks. | ||
| References | ||
|
REFERANCES
Aliev, A.S.; Dzhavadov, E.D. and Leont'eva, M.M. (1990): Indirect Hemagglutination test for infectious bursal disease of fowls. Vet. Bull., 60: 47-67.
Anjum, A.D.; Sabri, G.S. and Jamshidi, K. (1994): Occurrence spread and control of infectious bursal disease in Pakistan. In Proceedings of 1st PPAPVMA Punjab, International Poultry Conference March 30 - April 01, pp: 57-59.
Barnes, H.J.; Wheeler, J. and Reed, D. (1982): Serological evidence of infectious bursal disease virus infection in IOWA turkeys. Avian Dis., 26: 560-565.
Cao, Y.C.; YingZuo, B.I. and Zhu JiMei, (1995): Application of enzyme-linked immuno-sorbent assay for evaluation of immunological efficiency of chicks against IBD. Chinese J. Vet. Med., 21: 9-10.
Castello, L.; Martinez, B.L. and Heronodez, K. (1987): Comparison of the agar gel precipitation, virus neutralization and enzyme-linked immunosorbent assay in the determination of antibodies to infectious bursal disease virus. Veterinaria-Mexico, 18: 317-323.
Chettle, N.J. and Wyeth, P.J. (1989): Outbreak of virulent Infectious bursal disease in East Anglia. Vet. Rec. 125: 271-272.
Cullen, G.A. and P.J. Wyet, (1975): Quantitation of antibodies to infectious bursal disease. Vet. Rec., 93: 315.
Eterradossi, N.; Picault, J.P.; Druin, P.; Michele Guittlet; Rolande L'hospitalier, and Bennejean, G. (1992):Pathogenicity and preliminary antigenic characterization of 6 infectious bursal disease virus strains isolated in France from acute outbreaks. J. Vet. Med. B., 39: 683-691.
Hussain, I.; Nisar, A.; Rahman, S.U.; Mehmood, S.; Zahoor, A.; Ashfaque, M.; Mehmood, S. and Akhtar, M. (2002): Counter-immunoelectrophoresis-A rapid test for the detection and quantification of infectious bursal disease virus. Proc. (Abst). Regional Seminar on Prevalent and Newly emerging Poultry disease held on April 15-16, at University of Agriculture Faisalabad.
Hussain, I.; Zahoor, M.A.; Mehmood, M.S. and Akhtar, M. (2003): Single radial hemolysis technique in comparison with indirect hemagglutination and agar gel precipitation tests for assaying antibodies against infectious bursal disease virus. Pak. J. Life Soc. Sci., 1: 62-64.
Ikuta, N.; El-Atrache, J.; Villegas, P.; Garcia, M.; Lunge, VR.; Fonseca, ASK.; Oliveira, C. and Marques, EK. (2001): Molecular characterization of Brazilian Infectious Bursal Disease Viruses. Avian Diseases; 45 (2): 297-306.
Lukert, P.D. and Saif, Y.M. (1997): Infectious bursal disease. In: Calnek, B.W., Barnes, B.W., Beard, C.W., McDougald, L.R., Saif, Y.M. (Eds.), Diseases of Poultry. 10th ed. Iowa State University Press, Ames, pp. 721–738.
Lukert, P.D. and Saif, Y.M. (2003): Infectious bursal disease. In: Y.M. Saif (Ed.) Disease of Poultry.11th Ed., IowaStateUniversity, Ames, Iowa, USA, pp: 161-179.
Nakamura, T.; Otaki, Y. and Nunoya, T. (1992): Immunosuppressive effect of a highly virulent Infectious bursal disease virus isolated in Japan. Avian Dis., 36: 891-896.
Nicholas, R.A.J.; Reed, N.E.; Wood, G.W.; Herbert, C.N.; Muskett, J.C. and Thronton, D.H. (1985): Detection of antibodies against Infectious bursal disease virus: a comparison of three serological methods. Res. In Vet. Sci., 38: 189-192.
OIE (2004): Manual of recommended diagnostic techniques and requirements for biological products for lists A and B diseases. Office International Des Epizootes. Rahman, S.U.; Ashfaque, M. and Javed, S. (1994): Infectious bursal disease virus antibody titration using indirect Hemagglutination test. Pak. Vet. J. 14: 101-103.
Rautenschlein, S.; Yeh, H.Y. and Sharma, J.M. (2003): Comparative immunopathogenesis of mild, intermediate, and virulent strains of classic infectious bursal disease virus. Avian Diseases 47: 66-78. Saif, Y.M. (1991): Immunosuppression induced by infectious bursal disease virus. Vet. Immunol. Immunopath, 30: 45-5.
Sil, G.C.; Das, P.M.; Islam, M.R. and Rahman, M.M. (2002): Management and disease problems of Cockrels in some farms of Mymensingh, Bangladesh. Int. J. Poult. Sci., 1: 102-105.
Steel, R.G.D. and Torrie, J.H. (1960): Principles and procedures of statistics. McGraw-Hill Book Comp. Inc. New York, Toronto, London, pp. 99-131.
Tariq, J. (1999): Vaccines and Vaccination. AVN, Sep. 25, pp: 22-23.
Van Den Berg, T.P. and Meulemans, G. (1991): Acute infectious bursal disease in poultry protection afford by maternally derived antibodies and interference with live vaccination.Avian Pathol., 20, 3: 409-421.
Vui, T.Q.; Lohr, J.E.; Kyule, M.N.; Zessin, K.H. and Baumann, M.P.O. (2002): Antibody levels against Newcastle disease virus, Infectious bursal disease virus and Influenza virus in rural chicks in Vietnam. Int. J. Poult. Sci., 1: 127-132.
Wood, G.W.; Muskett, J.C. and Thornton, D.H. (1981): The interaction of live vaccine and maternal antibody in protection against infectious bursal disease. Avian Pathol., 10: 365-373. | ||
|
Statistics Article View: 147 PDF Download: 162 |
||
