PREGNANCY RATE IN EGYPTIAN BUFFALOES AFTER SYNCHRONIZATION OF ESTRUS WITH PGF2, OR OVULATION BY OVSYNCH PROGRAM

Assiut Vet. Med. J. Vol. 52 No. 111 October 2006

Animal Reproduction Research Institute, AL-Haram, Giza, Egypt.

PREGNANCY RATE IN EGYPTIAN BUFFALOES AFTER SYNCHRONIZATION OF ESTRUS WITH PGF2, OR OVULATION BY OVSYNCH PROGRAM

(With 2 Tables and 2 Figures)

By F.A. EL ZOHERY; A.A. NASRA and S.E.M. AL GHANDOUR

(Received at 20/9/2006)

تقييم معدل الحمل في الجاموس المصري في فترة مابعد الولادة باستخدام

الحقن المزدوج للبروستاجلاندين أو نظام تزامن التبويض

فوزي الزهيري ، عبد الخالق نصرة ، صلاح القدور تحول استطالة الفترة بين الولادتين في الجاموس المصري من زيادة الکفاءة الانتاجية له مما يؤثر سلبا على اقتصاديات انتاجه الأمر الذي حدی بالبحث عن وسائل متعددة لزيادة الخصوبة به وعلية تم اختيار عدد ۸ جاموسة حلابة، قسمت الى ثلاث مجموعات: المجموعة الأولى: حقنت في العضل بهرمون البروستاجلاندين مرتين بينهم 14 يوم، ثم تم

حقن الحيوانات التي لم تأتي في الشياع بجرعة ثالثة ثم التلقيح الاصطناعي بعد +۷۲-۸۰ ساعة. المجموعة الثانية: أستخدم معها الحقن المحرر للحاثة المنسلية- البروستاجلاندين - المحرر للحاثة أما المجموعة الثالثة فکانت عينة ضابطة. ولقد کانت نتائج المجموعة الثانية أعلى في نسبة الحمل من المجموعتين الأخرتين.

SUMMARY

Postpartum in buffaloes plays a great role in delay of the cyclicity in buffaloes that seemed to be anestrous. So, the use of hormones to induct estrus or induct ovulation has an importance to increase pregnancy rate. The present study was designed to evaluate the efficacy of the two methods in inducing high pregnancy rate. The experimental animals (48 lactating buffaloes) were divided into three groups: PGFza group 1(n=16); Ovsynch group 2 (n=16) and control group (n=16). Pregnancy rate was significantly (p<0.001)increased in group 2 than group 1 and control group due to higher level of progesterone and more accurate synchronization for ovulation., It is more suitable to use the regimen of ovsynch in postpartum buffaloes especially in multiparous females with good condition score.

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C-Blood Samples: Blood samples were collected on days 0 and 25 after insemination from the jugular vein by venipuncture into 10 ml vacutainer tubes. Serum was separated and stored at -20 °C until Progesterone hormone assay. D- Hormonal assay:

Progesterone was measured by direct radio immunoassay (RIA) using coat A count kit (Diagnostic Products Corporation) DPC. E- Pregnancy diagnosis:

Progesterone >1.0 ng/ml on day 25 post insemination was considered as pregnant and confirmed 60 days after the insemination by rectal palpation. F- Statistical analysis:

The data were statistically analyzed according to Snedecor and Cochran (1980). A Chi-square test was used to compare pregnancy rates to TAI (ovsynch) versus induced estrus with PGF2

RESULTS Group I (PGFza group), 4/8(50%) primiparous buffaloes responded and became pregnant (2, 1 and 1 pregnant buffalo after the 1st, 2nd and 3rd injections respectively), 5/8 (62.5%) multiparous buffaloes became pregnant (2, 2 and 1 after the 1st, 2nd and 3rd injections, respectively) with high significance (P <0.001) as shown in Table 1.

On the other hand pregnancy rates in (Group II, Ovsynch group), were 4/8 (50%) primiparous buffaloes (1 and 3 after injection of PGFza and 2 injection of GnRH, respectively), 6/8 (75%) multiparous buffaloes became pregnant (1, 2 and 3 after injection of 1st injection of GnRH, PGFza injection and 21 injection of GnRH, respectively) with high significant (P<0.001).

Group III (control group) for primiparous and multiparous buffaloes showed pregnancy rate 3/8 (37.5%) and 4/8 (50%) respectively as shown in Table 1.

The overall of pregnancy rates were 9/16 (56.25%) in PGF24 group, 10/16 (62.5% in ovsynch group and 7/16 (43.75%) in control group with a high significant (P< 0.001) increase in ovsynch group rather than control group and PGF 2a group as shown in Table 1.

tal 56.25

(518)

(91163

Table 1: Pregnancy rates in different groups (%)

Animal Group Primiparous Multiparous

GroupI

50.0

62.5 (PGF2a)

(418) Group II

50.0

75.0 (Ovsynch)

(418)

(618) Group III

37.5

50.0 (Control)

244

62.5 (10116) 4

43.75 (7116)

(318)

(418)

Assiut Vet. Med. J. Vol. 52 No. 111 October 2006

Total rates with different capital letters superscript in the last column differs significantly (P <0.001).

Pregnancy rates with different small letters superscripts in the same column differs significantly (P <0.001). Progesterone concentration:

As presented in Table (2) serum concentration of progesterone were not different for primiparous buffaloes in Groups I, II and III just before treatment (0.686 + 0.115, 0.755 + 0.076 and 0.676 + 0.045 ng/ml respectively,) while, in multiparous buffaloes in Groups I, II and III were (0.927 +0.157,0.722 + 0.058 and 0.767 + 0.085 ng/ml, respectively).

Regarding progesterone level after 25 days post treatment, it is found that the group II has a higher significant increase (P <001) (4.886 + 0.333 ng/ml) for primiparous and (5.083 I 0.100 ng/ml) for multiparous buffaloes; than group I (4.684 + 0.126, 4.734 +0.230ng/ml); and group III (4.837+ 0.353, 4.239 = .124 ng/ml) primiparous and multiparous pregnant animals, respectively.

Table 2: Serum progesterone profile for different groups (ng\ml)

(Mean = SEM)

Animal groups

Pretreatment (on day 0)

Post insemination (on day 25)

Primiparous

Multiparous

Non-Pregnant Primiparous Multiparous

N=4

N=3 1.118 Ba

0.865Cb

Pregnant Primiparous | Multiparous

N=4

N=5

N=8 0.6866

N=8 0.9272

GI (PG-PG)

4.684 Bb

4.734 Ba

+

0.115

N=8 0.7555

0.157

N=8 0.722

0.076 N=4

0.288

N=2 1.139 Bb

0.126 N=4

0.230 N=6

2.4734a

4.8864

5.0834a

GII

(Ovsynch)

0.076

N=8 0.676

0.058

N=8 0.767a

0.448 N=5

0.044 N=4

0.333 N=3

0.100 N=4

0.903

1.385A

4.8374

4.23900

G III (Control)

0.045

0.085

0.042

0.096

0.353

0.124

Progesterone levels (on day 0 with different small alphabetical superscripts in the same column differs significantly (P <0.001).

Progesterone levels with different capital alphabetical superscript in the same column differ significantly at least at p < 0.001. while, progesterone level (either in pregnant or in the non- pregnant group in the same raw) with different small alphabetical superscript differ significantly at least at (p <0.01).

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Pregnancy rate

2

Primiparous Multiparous Total

PG-PG group

GPG group

Control group

ne Pregnancy rates (%) in different groups.

Progesterone profile

ng/ml 2

Pre-treatment Post-treatment

PG-PG group

GPG group

Control group

erum progesterone profile (ng/ml) in different groups.

As shown in figure 1 pregnancy rate was higher in group II than other groups, while Figure 2 showed progesterone profile was higher comparatively in group II than other groups.

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DISCUSSION

The main purpose of this study was to evaluate the effect of PGF 2a as a way of synchronization of estrus and the Ovsynch program for ovulation synchronization with pregnancy rates in Egyptian buffaloes. PGF2c group resulted in 56.25% pregnancy rate in lactating buffaloes these results corresponded to the results in cows by Lucy et al., (1986); Stevenson et al. (1987) and Archbald et al. (1992) that possibly due to the variability in time from AI to ovulation. This variability in time from injection of PGFza to estrus and subsequent ovulation may be directly related to the number and size of the ovulatory follicles at the

TIU WC

pregnancy rate 62.5% which is considered high significance (P<0.001) rather than other groups (PGFza group and control group),this result agreed with those applied on Brazilian buffaloes by Roy et al. (1996); Burke et al. (1996); Momcilovic et al. (1998); Berber et al. (2002); Bartolomeu et al. (2002) and Paul and prakash (2005). A high percentage of pregnancy rate in lactating buffaloes (75%) at a random stage of the estrus cycle ovulated a follicle after first injection of GnRH specially in multiparous rather than primiparous that was in accordance with that results in cows revealed by Silcox et al. (1993). The difference between pregnancy rates of primiparous (50%, 50%, 37.5%) and multiparous (62.5%, 75%, 50%) in the different groups respectively agreed with Pietro et al. (2003) and demonstrated that the parity is a decisive factor in the efficiency of the protocols. Also, they reported

that we

snend energy for the continuity of the corporal growth

and multiparous converge that energy for the reproductive processes.

In regarding to plasma progesterone concentrations of pregnant buffaloes in groups and II tended to be higher in multiparous buffaloes 4.734+0.230 ng/ml and 5.083+0.100 ng/ml than in multiparous of group 3(4.239 +0.124ng/ml while they are not significant to the corresponding levels of the primiparous buffaloes 4.684 + 0.126 ng/ml and 4.886 +0.333 ng/ml in group I and II respectively that agreed with Mee, et al. (1993). This may be attributed to high level of progesterone concentrations of GPG buffaloes were probably due to the stimulatory effects of GnRH, or to prolonged effects of GnRH on the proportion of large luteal cells in the developing corpus luteum during pregnancy. So, estrus expression is limited with PGFza than GPG protocol based on fixed-time insemination protocols in lactating buffaloes and necessitates

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minimal amounts of estrus detection in order to reach maximal pregnancyrates.

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