Sarg, T., Zenk, M., El-Dahmy, S., Abdel-Ghani, A., Abou-Hashem, M. (2002). HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF COLCHICINE AND RELATED COMPOUNDS. test, 11(2), 64-70. doi: 10.21608/zjps.2002.178989
Taha Sarg; Meinhart Zenk; Sameih El-Dahmy; Afaf Abdel-Ghani; Maged Abou-Hashem. "HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF COLCHICINE AND RELATED COMPOUNDS". test, 11, 2, 2002, 64-70. doi: 10.21608/zjps.2002.178989
Sarg, T., Zenk, M., El-Dahmy, S., Abdel-Ghani, A., Abou-Hashem, M. (2002). 'HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF COLCHICINE AND RELATED COMPOUNDS', test, 11(2), pp. 64-70. doi: 10.21608/zjps.2002.178989
Sarg, T., Zenk, M., El-Dahmy, S., Abdel-Ghani, A., Abou-Hashem, M. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF COLCHICINE AND RELATED COMPOUNDS. test, 2002; 11(2): 64-70. doi: 10.21608/zjps.2002.178989

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF COLCHICINE AND RELATED COMPOUNDS

Zagazig Journal of Pharmaceutical Sciences
Article 8, Volume 11, Issue 2, December 2002, Pages 64-70    PDF (3.12 M)
Document Type: Original Article
DOI: 10.21608/zjps.2002.178989
Authors
Taha Sarg* 1; Meinhart Zenk2; Sameih El-Dahmy1; Afaf Abdel-Ghani3; Maged Abou-Hashem4
1Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Egypt.
2Institute of Pharmaceutical Biology, Munich University, Munich, Germany
3Dept. of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig , Egypt
4Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
Abstract
An HPLC method was applied for the determination of colchicine and colchicoside in the methanolic extract of the seeds of Colchicum autumnale L. during different stages of seed maturity. The method is carried out isocratically on a reversed-phase column using H2O-CH3CN (95:5) +100 µl H3PO4. The detection limits were 0.10 and 0.05 µg for colchicine and colchicoside, respectively and the recoveries (99.2 ± 1.1%) were high and quantitative. Also, a highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantitative determination of colchicine, its structurally related compounds (colchicinoids) in seeds of C. autumnale and suspension culture of Colchicum variegatum L. A combined experiment (HPLC- ELISA) was performed for detection of colchicine and some other colchicinoids in C. autumnale seeds extract.
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