Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83 | ||
| Archives of Pharmaceutical Sciences Ain Shams University | ||
| Article 12, Volume 5, Issue 1, June 2021, Pages 158-170 PDF (773.42 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/aps.2021.76831.1061 | ||
| Authors | ||
| Noha S. Elsayed* 1; Khaled M. Aboshanab1; Mahmoud A. Yassien1; Mohammad M. Aboulwafa2; Nadia A. Hassouna1 | ||
| 1Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt | ||
| 21. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University 2. Faculty of Pharmacy, King Salman International University, Ras-Sedr, South Sinai, Egypt | ||
| Abstract | ||
| Background: Polyhydroxyalkanoates (PHA) are environmentally friendly polymers produced by many bacteria under nutrient-limited conditions. However, their commercialization is hindered by the production expenses. The present study aimed at cost-effective and efficient production of poly(3-hydroxybutyrate) (PHB) by Bacillus (B.) cereus isolate CCASU-P83. Methods: Through one factor at time optimization study on shake flask, B. cereus CCASU-P83 produced about 50 % polymer per dry weight after 48 hours incubation time. For better evaluation of the fermentation process, kinetic modeling using the Logistic and Leudking-piret models was applied. Results: A preliminary economic analysis was carried out and leads to a 30.8 % reduction in the total cost. In comparison to the findings obtained on the level of shake flask, scaling up to the bioreactor resulted in producing about 53% PHB per dry weight after only 24 hours incubation. These models concluded that B. cereus produced PHB during the growth phase. Analysis of molecular weight of the produced polymer displayed a 26900 g/mole molecular weight with a polydispersity index (PDI) of 1.1. Conclusion: B. cereus CCASU-P83 is a potential candidate for industrial production of PHB polymer using corn oil in a short incubation period which highly reduced the cost of the production process. | ||
| Keywords | ||
| Polyhydroxyalkanoates; Logistic; Leudking; Fermentation; optimization | ||
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