Enhancement of bioethanol production by Saccharomyces cerevisiae under high gravity fermentation using sequential optimization strategy | ||
| Egyptian Journal of Chemistry | ||
| Article 15, Volume 64, Issue 12, December 2021, Pages 7017-7028 PDF (537.95 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2021.78360.3904 | ||
| Authors | ||
| Heba E Mohammed1; Mohamed I. Abo-Alkasem* 2; Ehab M. Ammar3; Tarek E. Mazeed4; Amira M Abu Taleb5 | ||
| 1Distillation Factories, Egyptian Sugar and Integrated Company, El-Hawamdia Giza, Egypt | ||
| 2Chemistry of natural and microbial products, Pharmaceutical research division. National Research Centre, Giza, Egypt | ||
| 3Genetic Engineering and Biotechnology Research Institute, University of Sadat City, El-Sadat City, Egypt Patel College of Global Sustainability, University of South Florida, 4202 E. Fowler Avenue, Tampa, FL 33620, USA | ||
| 4Chemistry of Natural and Microbial Products Dept., Pharmaceutical and Drug Industries Research Div., National Research Centre, Dokki, Giza, Egypt Department of Chemical and Bimolecular Engineering, Ohio State University, United State of America. | ||
| 5Botany and Microbiology Department, Faculty of Science. Cairo University, Egypt. | ||
| Abstract | ||
| Using high gravity fermentation for bioethanol production by Saccharomyces cerevisiae is faced with many challenges such as increasing osmotic stress and viscosity. Unfortunately, increasing specific gravity was accompanied by a decrease in fermentation efficiency. A two-stage sequential optimization strategy was carried out to solve this problem. The first stage was carried out using the Plackett-Burman Design in which eight factors were investigated at three molasses specific gravities (1.130, 1.145, 1.160). Urea, wheat bran, soy flour, and inoculum size were significant model terms and had positive effect on bioethanol production. When a validation test was performed using the predicted conditions, about a more than two-fold increase in fermentation efficiency (FE) was achieved (83%) at specific gravity 1.130, compared to the basal condition. The second stage (Taguchi Design) was conducted using eight factors. Urea, wheat bran, temperature, and agitation speed showed a significant effect on fermentation efficiency. A validation test was carried out and up to 83.82 % fermentation efficiency was recorded, which was very close to that achieved under normal gravity. In addition to the high value of FE, this strategy is cost-effective and time-saving. | ||
| Keywords | ||
| bioethanol; molasses; S. cerevisiae; high gravity fermentation; statistical design | ||
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