Ameliorating Role of Nitric Oxide in Germinating Mung Bean Seeds (Vigna Radiata) Under Lead Stress | ||
| Catrina: The International Journal of Environmental Sciences | ||
| Article 10, Volume 8, Issue 1, 2013, Pages 75-85 PDF (386.82 K) | ||
| Document Type: Original Article | ||
| DOI: 10.12816/0010677 | ||
| Author | ||
| Hala Aly* | ||
| Botany Department, Faculty of Science, Alexandria University, 21511, Alexandria, Egypt | ||
| Abstract | ||
| Protective role of nitric oxide (NO) on seed germination and growth was studied in mung bean seeds (Vigna radiata) subjected to lead (Pb) stress. Germination percentage was increased up to 50 μM sodium nitroprusside (SNP) and then steady declined. Under lead stress, and absence of SNP, percentage of germination, fresh biomass, succulent value and the activity of α-amylase and protease as well as HATPase were significantly decreased. This was accompanied with a significant increase of hydrogen peroxide and malondialdhyde accumulation, catalase and guiacol peroxidase activities. Furthermore, total phenolic compounds as well as some individual phenolic acids were increased in a dose-dependent manner of lead. The protein pattern showed appearance of stress protein with M. M. of 110, 97 and 21 KDa at various lead stresses. Moreover, supplementation of SNP, as source of NO, increased fresh biomass, succulent values and enhanced the activities of H-ATPase, α- amylase, protease, catalase and guiacol peroxidase in lead stressed germinating bean seeds, whereas reduced the lead-induced increase in lead, hydrogen peroxide, malondialdhyde and phenolics accumulation. A new stress protein with M.M. 65 KDa was appeared in all lead stressed seedlings in presence of SNP. Taken together these results suggest that SNP could increase antioxidant capability in mung bean seeds leading to an improvement in the plasma membrane integrity and enhancement of the activities of hydrolytic enzymes and hence, an ameliorating role against the inhibitory impact of this toxicant on the germination and growth of mung bean seeds under lead stress | ||
| Keywords | ||
| α -Amylase; Antioxidant enzymes; H-Atpase; Lead; Mung Bean; Phenolic compounds; Protease; SNP | ||
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