Gad, M., El Fiky, B., El-Saiedy, H., Sabra, E. (2021). ACTIVITY STUDY OF FRESH AND FROZEN-THAWED SPERMATOZOA ON INTRACYTOPLASMIC SPERM INJECTION OUTCOME. test, 26(2), 311-323. doi: 10.21608/jpd.2021.181335
Mohamed Gad; Bahgat El Fiky; Hanan El-Saiedy; Ebrahim Sabra. "ACTIVITY STUDY OF FRESH AND FROZEN-THAWED SPERMATOZOA ON INTRACYTOPLASMIC SPERM INJECTION OUTCOME". test, 26, 2, 2021, 311-323. doi: 10.21608/jpd.2021.181335
Gad, M., El Fiky, B., El-Saiedy, H., Sabra, E. (2021). 'ACTIVITY STUDY OF FRESH AND FROZEN-THAWED SPERMATOZOA ON INTRACYTOPLASMIC SPERM INJECTION OUTCOME', test, 26(2), pp. 311-323. doi: 10.21608/jpd.2021.181335
Gad, M., El Fiky, B., El-Saiedy, H., Sabra, E. ACTIVITY STUDY OF FRESH AND FROZEN-THAWED SPERMATOZOA ON INTRACYTOPLASMIC SPERM INJECTION OUTCOME. test, 2021; 26(2): 311-323. doi: 10.21608/jpd.2021.181335

ACTIVITY STUDY OF FRESH AND FROZEN-THAWED SPERMATOZOA ON INTRACYTOPLASMIC SPERM INJECTION OUTCOME

Journal of Productivity and Development
Article 7, Volume 26, Issue 2, April 2021, Pages 311-323    PDF (476.83 K)
Document Type: Original Article
DOI: 10.21608/jpd.2021.181335
Authors
Mohamed Gad1; Bahgat El Fiky* 2; Hanan El-Saiedy2; Ebrahim Sabra3
1Obs.& Gyn Department, Faculty of Medicine, Menoufia University, Egypt
2Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt
3Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
Abstract
To assess fertilization, implantation, and pregnancy rates in patients undergoing intracytoplasmic sperm inject (ICSI) using fresh and cryopreserved sperm from ejaculated semen samples.Retrospective study, from a total of 60 patients, 44 ICSI cycles were included in this study.Fertilization, implantation, and pregnancy rates were evaluated; 20 cycles of ICSI (cryopreserved-sperm group) and 20 cycles of ICSI (fresh-sperm group). Also, divided the outcomes according to the semen characteristics, normozoospermia, oligozoospermia, asthenozoospermia, and oligoasthenozoospermia.Overall, normal-fertilization rates were higher using fresh sperm compared with cryopreserved sperm.
 Cycles performed in patients with normozoospermia or oligozoospermia had similar fertilization, implantation, and pregnancy rates using fresh or cryopreserved sperm. When asthenozoospermic and oligoasthenozoospermic semen samples were used, the normal-fertilization rate was higher with fresh sperm compared with cryopreserved sperm. However, implantation and pregnancy rates were similar in fresh and cryopreserved sperm samples from patients with asthenozoospermia or oligoasthenozoospermia.
In conclusion, semen with abnormalities in the motility may be more susceptible to sperm cryopreservation damage, resulting in lower fertilization rates. However, once the oocyte is fertilized, implantation and pregnancy rates are similar to those in patients with oligozoospermia and normozoospermia.
       
 
 
 
Keywords
Semen; cryopreservation; ICSI; spermatozoa; fertilization
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