In Vitro and In Silico Analysis for Antibacterial Activitiy of Various Extracts of Gracilaria salicornia (Rhodophyta) from Selayar Islands, Indonesia | ||
| Egyptian Journal of Chemistry | ||
| Article 35, Volume 64, Issue 12, December 2021, Pages 7203-7212 PDF (435.15 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2021.69269.3540 | ||
| Authors | ||
| Bahrun bahrun; Nunuk Soekamto* ; Firdaus Firdaus | ||
| Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University | ||
| Abstract | ||
| This study aims to evaluate the toxicity, antibacterial activity, and chemical profiling of Gracilaria salicornia extract. The research stages included the extraction, bioactivity test, GC-MS, and docking analysis. The antibacterial activity showed better sensitivity against Escherichia coli than Staphylococcus aureus. The inhibition zone against E. coli ranged from 7.73-17.30 mm, while S. aureus ranged from 6.91-13.44 mm, and all of the extracts were toxic to Artemia salina Leach. The chloroform extract showed the highest activity with the clear zone diameter was 17.3±1.65 mm and 13.44 ± 0.04 mm to E. coli and S. aureus respectively. The presence of steroids, terpenoids, fatty acids, and alkane based on GC-MS and docking analysis were responsible for these activities. Molecular docking analysis showed that Cholest-5-en-3-ol and Cholesta-4,6-dien-3-ol showed better interactions than control with binding energy -9.99 and -9.67 kcal.mol-1 against 5BMM, -8.00 and -7.93 kcal.mol-1 against 3LPS respectively. Based on this data, it can be stated that G. salicornia extract is highly potential as the source of antibacterial compounds. | ||
| Keywords | ||
| Antibacterial activity; Binding energy; Docking; Gracilaria salicornia; GC-MS; and Toxicity | ||
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