The effects of folic acid on carbon black toxicity in mouse embryo in vivo | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 7, Volume 14, Issue 1, January 2004, Pages 68-78 PDF (322.38 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejhm.2004.18222 | ||
| Authors | ||
| Roshdy, H.M; Bibars, M.H | ||
| Cell Biology Department, National Research Center Dokki, Cairo, Egypt | ||
| Abstract | ||
| The wide commercial use of carbon black oil (CBO) to produce asphalt and other commercial product has resulted in numerous environmental problems and harmful effects on human health especially during the pregnancy. This study, examining the effect of maternal low and high dietary folate intake and to protect the pregnant women from the developmental toxicity of CBO. Virgin females CD—1 mice were assigned to diets containing either low 500 or 1300 high (control) nmol folic acid/kg for 6 weeks prior to mating and thought out breeding and gestation. From gestation day (GD 6 to 18) females were given by gavage corn oil or CBO at 500 mg/kg body weight, once daily. On CD 18, mice were weight and killed and the liver removed and weighed. Implantation sites, live and dead fetuses, and resorptions were counted, fetuses were weighed individually and examined for external malformations. The low dietary FA treatment alone and with CBO treatment resulted in low maternal liver as well as low fetal liver folate concentrations relative to the high FA dietary groups. Low FA treatment alone resulted in malformed embryos; there were no embryos affected with malformed in the adequate FA-control group. Low folic acid-CBO treatment resulted in a further increase in the malformed embryos. The percent of malformed embryos in high folic acid-CBO treatment was very low compared to the low folic acid-CBO group. The frequency of chromosomal aberrations in maternal and their fetuses was increased significantly in the low folic-CBO group than high folic acid-CBO group. These results show that the low folate dietary diet with the exposure to the high levels of CBO toxic material in pregnant women significantly increases the developmental and mutagenic toxicity in the small fetuses. | ||
|
Statistics Article View: 155 PDF Download: 393 |
||
