Mohamed, T., Refahy, L., Romeih, M., Hamed, M., Ahmed, H., El-Hashash, M. (2022). Chromatographic Isolation and Characterization of Certain Bioactive Chemical Ingredients of Phyllanthus emblica Extracts and Assessment of Their Potentials as Antiviral and Anticancer Agents. test, 65(1), 179-192. doi: 10.21608/ejchem.2021.79403.3902
Tamer Samy Mohamed; Laila Abdel-Ghany Refahy; Mahmoud Hassan Romeih; Manal Mortady Hamed; Hend Okasha Ahmed; Maher Abdel-Aziz El-Hashash. "Chromatographic Isolation and Characterization of Certain Bioactive Chemical Ingredients of Phyllanthus emblica Extracts and Assessment of Their Potentials as Antiviral and Anticancer Agents". test, 65, 1, 2022, 179-192. doi: 10.21608/ejchem.2021.79403.3902
Mohamed, T., Refahy, L., Romeih, M., Hamed, M., Ahmed, H., El-Hashash, M. (2022). 'Chromatographic Isolation and Characterization of Certain Bioactive Chemical Ingredients of Phyllanthus emblica Extracts and Assessment of Their Potentials as Antiviral and Anticancer Agents', test, 65(1), pp. 179-192. doi: 10.21608/ejchem.2021.79403.3902
Mohamed, T., Refahy, L., Romeih, M., Hamed, M., Ahmed, H., El-Hashash, M. Chromatographic Isolation and Characterization of Certain Bioactive Chemical Ingredients of Phyllanthus emblica Extracts and Assessment of Their Potentials as Antiviral and Anticancer Agents. test, 2022; 65(1): 179-192. doi: 10.21608/ejchem.2021.79403.3902

Chromatographic Isolation and Characterization of Certain Bioactive Chemical Ingredients of Phyllanthus emblica Extracts and Assessment of Their Potentials as Antiviral and Anticancer Agents

Egyptian Journal of Chemistry
Article 20, Volume 65, Issue 1, January 2022, Pages 179-192    PDF (571.44 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.79403.3902
Authors
Tamer Samy Mohamed* 1; Laila Abdel-Ghany Refahy2; Mahmoud Hassan Romeih3; Manal Mortady Hamed4; Hend Okasha Ahmed5; Maher Abdel-Aziz El-Hashash6
1Laboratory of Medicinal Chemistry, Theodor Bilharz Institute, Korniash El-Nile, 12411 Warrak El-Hadar, Giza, Egypt.
2Medicinal Chemistry Department, Theodor Bilharz Research Institute (TBRI)
3Laboratory of Biochemistry and molecular biology, Theodor Bilharz Research Institute
4Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt
5bLaboratory of Biochemistry and molecular biology, Theodor Bilharz Research Institute, Giza , Egypt
6Department of Chemistry, Faculty of Science, Ain-Shams University, Abasia, Cairo, Egypt.
Abstract
Aim: our study has been aimed to evaluate the antioxidant, anticancer and antiviral activities of Phyllanthus emblica leaves, as well as characterization of bioactive chemical constituents. Methods: The total phenolic and flavonoid contents of plant extracts were determined using Folin-Ciocalteu’s and aluminium trichloride assays, respectively. The antioxidant activities were evaluated by different methods as well as the anticancer activity via MTT assay, while the antiviral activity was evaluated using cytopathic effect (CPE) inhibition and anti- HCV assays. Moreover, the extracts phytoconstituents were characterized using reversed phase high-performance liquid-chromatography method. Results: Our finding revealed that P. emblica is rich in glycosides, flavonoids, phenolics, terpenoids and tannins. The n-butanol fraction exhibited the highest total phenolic and flavonoid contents with values 654.78 mg GAE /g ext and 110.14 mg RE / g ext, respectively. Also, it possessed the most effective antioxidant activities (DPPH; SC50=19.59 µg/mL, RPAA; 0.948 mg AAE /g ext., and TAC; 543.62mg AAE /g ext.) and anticancer activity against HepG-2 and MCF-7 cell lines with an IC50 of 25.63 and 22.80μg/mL, respectively. Moreover, Phyllanthus emblica extracts exhibited anti-HCV activity. The HPLC finger print results of Phyllanthus emblica extracts showed 14 peaks superimposed to the standards. Structure elucidation of pure isolates was achieved via IR, UV, 1H & 13C-NMR
Keywords
Phyllanthus emblica; HPLC; Antioxidants; Antiviral; Anticancer
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