IMPACT OF GROWTH HORMONE GENE TRANSFER BY SPERM-MEDIATED GENE TRANSFER ON EGG PRODUCTIVE AND REPRODUCTIVE TRAITS OF BANDARAH LOCAL CHICKEN | ||
| Egyptian Poultry Science Journal | ||
| Article 10, Volume 41, Issue 2, June 2021, Pages 413-428 PDF (735.09 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/epsj.2021.182742 | ||
| Authors | ||
| waleed salah El-Tahawy1; Hanan hassan Ghanem2 | ||
| 1ANIMAL AND POULTRY PRODUCTION .Fac.of Agric. Damanhour Unvi. | ||
| 2Egypt | ||
| Abstract | ||
| The experimental was aimed to improve genetic of Bandarah Local chicken strain to insert Growth hormone gene (cGH) from (Cobb 500) broiler strain by method sperm-mediated gene transfer technique (SMGT). This study investigated for two generations. Total RNA was extracted from chicken liver tissue and the cDNA was successfully prepared. PCR Products GH, mRNA normal Length 810bp for transgenic Bandarah chickens which treated by SMGT method, the same result was found in the first and second generation. The averages of fertility percentage were 88.97% and 92.14 % for SMGT and control, respectively. The overall mean of the second generation was nearly value 90.76% of the first one 90.38%.The responses of the transgenic techniques SMGT for fertility percentage were 5.64%. The responses of hatchability for SMGT were 18.35% and 21.82% for fertile and total eggs, respectively The SMGT of transferring was decreased the age at sexual maturity ASM at the second generation by 35.01 d. Egg number which produced during the first 90 days from SMGT method significantly increased compared by the control. There was highly significant difference between treated found, the SMGT method had the heaviest egg weight 49.20 g followed by control one 48.22 g. After two generations, the SMGT technique was improved egg number by 10.62 egg, decreased egg weight by 0.9g and improved egg mass during the first 90 days of laying by 512.62 g. SMGT is an efficient method that will hopefully facilitate the implementation of strategies for securing the benefits that can be expected to arise from the introduction of transgenic chicken. Chicken cGH gene was affected in egg productive traits and reproductive traits and moved from the first generation to the second generation with the same shape and increased the effect. Growth hormone gene transfer by SMGT will save time to improve egg productive traits and reproductive traits. | ||
| Keywords | ||
| Egg traits; reproductive traits; GH gen; gene transfer; SMGT | ||
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