GROWTH STUDIES OF PANLEUKOPENIA VIRUS ON TISSUE CULTURE | ||
| Assiut Veterinary Medical Journal | ||
| Article 6, Volume 39.2, Issue 78, July 1998, Pages 80-91 PDF (3.03 M) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.1998.183196 | ||
| Authors | ||
| Attyat M. Kotb1; Y.Z. Habbashi1; M.S. Saber2 | ||
| 1Vet. Serum and Vaccine Research Institute, Abbasia, Cairo | ||
| 2Fac. of Vet. Med., Cairo Univ. | ||
| Abstract | ||
| Feline panleukopenia virus was propagated in different types of cell cultures from different origins and passages: NLFK 224, NLFK319, CRFK, A72, MDCK, Vero and BHK 21. It was found that the most suitable cells for virus propagation were NLFK224. The growth curve of this cell culture showed that the highest infectivity titre for cell free virus (CFV) was 10%. TCD50/ml (Tissue culture infective dose) after 72 hours post inoculation (PI), cell associated virus (CAV) 105.2 TCIDsd/ml 96 hours PI and total virus (TV) yield 10** TCD5o/ml 96 hours PI. The haemagglutination titre of CAV was 64/0.025ml 72 hours PI while that of CFV was 128/0.025ml 96 hours PI and that of TV reached 256/0.025ml 96 hours PI. The cytopathic effect of the virus was characterized by the appearance of intracytoplasmic inclusion bodies after 24 hours PI and intranuclear inclusion bodies after 72 hours PI. These results were confirmed by direct fluorescent antibody technique. | ||
| Keywords | ||
| Key Words: Panleukopenia Virus; Tissue Culture; Growth | ||
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