elwia, S., Marei, Y. (2021). Molecular Insights into The Biological Basis of Anticancer Efficacy of miRNA Suppression Therapy in HCC Cell Lines. test, 13(1), 173-182. doi: 10.21608/eajbsc.2021.183298
sania k. elwia; Yasmin M. Marei. "Molecular Insights into The Biological Basis of Anticancer Efficacy of miRNA Suppression Therapy in HCC Cell Lines". test, 13, 1, 2021, 173-182. doi: 10.21608/eajbsc.2021.183298
elwia, S., Marei, Y. (2021). 'Molecular Insights into The Biological Basis of Anticancer Efficacy of miRNA Suppression Therapy in HCC Cell Lines', test, 13(1), pp. 173-182. doi: 10.21608/eajbsc.2021.183298
elwia, S., Marei, Y. Molecular Insights into The Biological Basis of Anticancer Efficacy of miRNA Suppression Therapy in HCC Cell Lines. test, 2021; 13(1): 173-182. doi: 10.21608/eajbsc.2021.183298

Molecular Insights into The Biological Basis of Anticancer Efficacy of miRNA Suppression Therapy in HCC Cell Lines

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 14, Volume 13, Issue 1, June 2021, Pages 173-182    PDF (779.59 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2021.183298
Authors
sania k. elwia; Yasmin M. Marei
Department of Medical Biochemistry & Molecular Biology, Benha Faculty of Medicine, Benha University, Qalubyia, Egypt
Abstract
Background: MiRNAs are critical in the course and prognosis of the disease and may aid in developing innovative therapies. Due to miRNAs instability and complicated environment, which includes in vivo degradation by nucleases, deregulation of miRNA expression is the hallmark of liver cancer. MiRNAs can function as oncogenes or tumor suppressors; miRNA-222 promotes tumor progression by cancer-related biological processes. MiRNAs are a novel method for cancer gene therapy due to their influence on cell signaling pathways by inhibiting or promoting many related genes. The interaction between genetics and cellular pathways contributes significantly to the onset and progression of HCC
This study aims to illustrate molecular insights into the biological basis of potential anticancer efficacy of anti-microRNA in HCC cell lines
Results:Normalization of dysregulated miRNAs by down-regulation block HCC cell proliferation or increase the sensitivity of liver cancer cells to chemotherapy. Effect of UTMD-mediated with anti-microRNA-222 leads to cell proliferation inhibition and cell apoptosis induction in HepG2 cells line. Inhibition of miR222 expression leads to cell cycle arrest and activation of its target genes.
Conclusion: Ultrasound microbubbles are frequently utilized to investigate gene and miRNA functions. Gene delivery of miR-222 inhibitor by microbubbles/ultrasound is a new therapy and overcome chemotherapy side effects. 
Keywords
HCC; anti-miR-222; Ultrasound microbubbles; HepG2 cells; apoptotic genes
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