RADWAN, G., ATTIA, E., TAWFIK, M. (1997). RAPID DETECTION AND DIFFERENTIATION OF LEPTOSPIRA BY POLYMERASE CHAIN REACTION. test, 36.2(72), 326-335. doi: 10.21608/avmj.1997.183383
G.S. RADWAN; E.R.H. ATTIA; M.S. TAWFIK. "RAPID DETECTION AND DIFFERENTIATION OF LEPTOSPIRA BY POLYMERASE CHAIN REACTION". test, 36.2, 72, 1997, 326-335. doi: 10.21608/avmj.1997.183383
RADWAN, G., ATTIA, E., TAWFIK, M. (1997). 'RAPID DETECTION AND DIFFERENTIATION OF LEPTOSPIRA BY POLYMERASE CHAIN REACTION', test, 36.2(72), pp. 326-335. doi: 10.21608/avmj.1997.183383
RADWAN, G., ATTIA, E., TAWFIK, M. RAPID DETECTION AND DIFFERENTIATION OF LEPTOSPIRA BY POLYMERASE CHAIN REACTION. test, 1997; 36.2(72): 326-335. doi: 10.21608/avmj.1997.183383

RAPID DETECTION AND DIFFERENTIATION OF LEPTOSPIRA BY POLYMERASE CHAIN REACTION

Assiut Veterinary Medical Journal
Article 26, Volume 36.2, Issue 72, January 1997, Pages 326-335    PDF (2.78 M)
Document Type: Research article
DOI: 10.21608/avmj.1997.183383
Authors
G.S. RADWAN; E.R.H. ATTIA; M.S. TAWFIK
Abstract
The technique of arbitrarily primed polymerase chain reaction (AP-PCR) or random amplified polymorphic DNA (RAPD) fingerprinting was used for differentiation of leptospira. The assay allowed the rapid differentiation of standard: L.hardjo, L.pomona and L.canicola examined reference strains. In another experiment, this PCR assay successfully detected leptospiral DNA in L.hardjo-seeded bovine urine. The specificity of leptospiral DNA amplification from urine samples was confirmed by southern blot hybirdization using a nonradioactive labeled DNA probe. The data suggested that AP-PCR may be used for rapid differentiation and typing of leptospiral
isolates, and a valuable test for specific detection of leptospira in bovine field samples.
Keywords
Key words: Leptospira; Detection; PCR
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