Nematicidal Effect of Paeonia Suffruticosa Constituents | ||
| Alexandria Science Exchange Journal | ||
| Article 1, Volume 28, JANUARY- MARCH - Serial Number 1, January 2007, Pages 1-8 PDF (487.83 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/asejaiqjsae.2007.1837 | ||
| Author | ||
| Ahmed S. Abdel-Aty | ||
| Pesticide Chemistry Department, Faculty of Agriculture., Alexandria University | ||
| Abstract | ||
| Due to their harmful effects on the environmental phases, synthetic nematicides are now reappraised and the public concerns are tending to the natural nematicides as an alternative safe strategy for nematode control. The methanol extract of Paeonia suffruticosa leaves was tested for its activity at a concentration of 1000 ppm on the L1 larva and egg-bearing adult stages of Caenorhabditis elegans nematode and it proved to be active on both stages. The extract was fractionated to ten fractions by liquid partitioning followed by chromatographic separation on Sephadex LH-20 open column chromatography. The most active fraction (0.61 %) was hydrolyzed to its components with concentrated sulfuric acid and the resultant constituents were purified by thin layer chromatography (TLC) and paper chromatography (PC). These components were identified with 1H and 13C NMR spectroscopy to gallic acid and D-glucose, so the active constituent is a gallotannin derivative. | ||
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