Assessment of CD4+CD25+ Regulatory T Lymphocytes Cells in Chronic Hepatitis C, Chronic Hepatitis C– Associated Cirrhosis and Hepatocellular Carcinoma Patients | ||
| Catrina: The International Journal of Environmental Sciences | ||
| Article 1, Volume 7, Issue 1, 2012, Pages 1-7 PDF (157.14 K) | ||
| Document Type: Original Article | ||
| Authors | ||
| Mansour J1; Amal Abdel-Hai2; Mohamed EL din3; Ahmed E2; Samira Mansour* 4 | ||
| 1Clinical Laboratory Department, El-Ghad Faculty for Health Science, Jeddah, KSA. | ||
| 2Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. | ||
| 3Department of Internal Medicine, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. | ||
| 4Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt. | ||
| Abstract | ||
| CD4+CD25+ regulatory T lymphocytes are a subset of circulating CD4+ T cells with suppressive properties. CD4+CD25+ regulatory T cells suppress HCV-specific T cell responses and it has been suggested that they may play a role in viral persistence. Our aim was to assess the frequency of regulatory T cells in the different clinical presentations of hepatitis C virus infections in a chosen Egyptian population. Peripheral blood CD4+CD25+ regulatory T cells from patients with different progression of HCV, was conducted and analyzed using flow cytometry. For each stage of HCV progression, twenty patients (n=20) were subjected for analysis in comparison with normal healthy control subjects (n=20). Results showed a significantly higher frequency of CD4+CD25+ regulatory T cells in chronic HCV (3.0 ± 0.9%), HCV related liver cirrhosis (٣.٣±0.8%), HCV related-HCC (3.9 ±1.6%), when compared to normal healthy controls (2.3 ±0.8%). No statistical significant differences were found when comparing HCV related cirrhotic patients with chronic HCV infected patients (P=0.95). Meanwhile, statistical significant differences were detected when comparing HCV related HCC patients with chronic HCV patients (P=0.047). In conclusion, our results indicated the presence of significant higher than normal frequency of peripheral blood CD4+CD25+ regulatory T cell among Egyptian patients that may help to early detect the viral disease and open new field to find a way to disease recovery or protection. Conversely, within different clinical presentation of hepatitis C virus infection no significant differences were detected. Severity of liver affection was significantly correlated with viral load. | ||
| Keywords | ||
| Chronic hepatitis; Flow cytometry; Hepatitis C infection; Hepatitis associated cirrhosis; Hepatocellular carcinoma; liver disease; Regulatory T cells | ||
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