H., A., A., A., M., K., K., A. (2011). Micropropagation of Rumex vesicarius L. Through Shoot Tip Culture. test, 6(1), 41-45.
Abo El-soud H.; AL-azab A.; Koriesh M.; Atta-Alla K.. "Micropropagation of Rumex vesicarius L. Through Shoot Tip Culture". test, 6, 1, 2011, 41-45.
H., A., A., A., M., K., K., A. (2011). 'Micropropagation of Rumex vesicarius L. Through Shoot Tip Culture', test, 6(1), pp. 41-45.
H., A., A., A., M., K., K., A. Micropropagation of Rumex vesicarius L. Through Shoot Tip Culture. test, 2011; 6(1): 41-45.

Micropropagation of Rumex vesicarius L. Through Shoot Tip Culture

Catrina: The International Journal of Environmental Sciences
Article 5, Volume 6, Issue 1, 2011, Pages 41-45    PDF (1012.55 K)
Document Type: Original Article
Authors
Abo El-soud H.; AL-azab A.; Koriesh M.; Atta-Alla K.*
Horticulture Dept., Faculty of Agriculture, Suez Canal University, 41522, Ismailia, Egypt
Abstract
Shoot tips of Rumex vesicarius L. were excised from in vitro seedlings and transferred for shoot multiplication into MS medium supplemented with various concentrations of 6-benzyladenin (BA) or Kinetin (Kin). After 4 weeks, shoot multiplication was best achieved from explants cultured on MS medium containing 8.88 μM BA, with an average of 11.0 shoots per explant. While, the shoot tips cultured on the medium containing 26.63 μM Kin produced 4.6 shoots per explant. For rooting, the proliferated shoots were cultured on rooting medium containing IBA or NAA at different rates. Root induction was successfully occurred on MS medium supplemented with 2.46μM IBA or 7.38μM NAA .The survival rate of transplants during acclimatization reached 87% and the plants grew normally under the greenhouse conditions.
Keywords
Rumex vesicarius; micropropagation; 6-Benzyladenin (BA); Kinetin (Kin); Naphthalene Acetic Acid (NAA); Indole butyric acid (IBA)
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