Abdel-Salam, H., Gatzke, R., Serry, F., Abd El-Latif, H., Hollenberg, C. (1996). EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain. test, 5(2), 62-70. doi: 10.21608/zjps.1996.185017
Hassan Abdel-Salam; Ralf Gatzke; Fathy Serry; Hemat Abd El-Latif; Cornelis Hollenberg. "EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain". test, 5, 2, 1996, 62-70. doi: 10.21608/zjps.1996.185017
Abdel-Salam, H., Gatzke, R., Serry, F., Abd El-Latif, H., Hollenberg, C. (1996). 'EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain', test, 5(2), pp. 62-70. doi: 10.21608/zjps.1996.185017
Abdel-Salam, H., Gatzke, R., Serry, F., Abd El-Latif, H., Hollenberg, C. EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain. test, 1996; 5(2): 62-70. doi: 10.21608/zjps.1996.185017

EXPRESSION OF MOUSE ANTICREATINE KINASE (MAK 33), FAR FRAGMENT OF IgG ANTIBODY, IN THE METHYLOTROPHIC YEAST HANSENULA POLYMORPHA II. Gamma chain

Zagazig Journal of Pharmaceutical Sciences
Article 10, Volume 5, Issue 2, December 1996, Pages 62-70    PDF (5.6 M)
Document Type: Original Article
DOI: 10.21608/zjps.1996.185017
Authors
Hassan Abdel-Salam* 1; Ralf Gatzke2; Fathy Serry3; Hemat Abd El-Latif4; Cornelis Hollenberg2
1Department of Microbiology, Faculty of Pharmacy, Zagazig University
2Institut für Mikrobiologie, Heinrich-Heine Universität, Düsseldorf, Universitätsstr. Düsseldorf, Germany.
3Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt
4Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt
Abstract
The yeast Hapsenula pdymorpha was used as a host for expression of gamma chain peptide of FAb fragment of mouse articreatine kinase-M, IgG antibody. Two expression systems were designed and assembled based on the promoter codon of either of the preferentially highly expressed enzymes, formate dehydrogenase (FMD) and methanol oxidase (MOX) of H. polymorpha. Two expression plasmid vectors, PGAGU and PHAAI, with FMD and MOX promoters, representity, were contsructed and transformed into H. pdymorpha LR9 Expression of gamma chain gene yielded about 400 mg/L and 550 mg/L, respectively 7% and 10% of the total cell protein under the control of FMD and MOX promoters, respectively. The prepro alpha was incompletely processed and most of the gamma chain peptide accumulated within the cells, especially under the control of FMD promoter.
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