Sarg, T., Zenk, M., El-Dahmy, S., Abdel-Ghani, A., Abou-Hashem, M. (1996). APPLICATION OF IMMUNOENZYMATIC TECHNIQUE IN DETERMINATION OF GALANTHAMINE IN TWO PANCRATIUM SPECIES. test, 5(2), 99-104. doi: 10.21608/zjps.1996.185026
Taha Sarg; Meinhart Zenk; Sameih El-Dahmy; Afaf Abdel-Ghani; Maged Abou-Hashem. "APPLICATION OF IMMUNOENZYMATIC TECHNIQUE IN DETERMINATION OF GALANTHAMINE IN TWO PANCRATIUM SPECIES". test, 5, 2, 1996, 99-104. doi: 10.21608/zjps.1996.185026
Sarg, T., Zenk, M., El-Dahmy, S., Abdel-Ghani, A., Abou-Hashem, M. (1996). 'APPLICATION OF IMMUNOENZYMATIC TECHNIQUE IN DETERMINATION OF GALANTHAMINE IN TWO PANCRATIUM SPECIES', test, 5(2), pp. 99-104. doi: 10.21608/zjps.1996.185026
Sarg, T., Zenk, M., El-Dahmy, S., Abdel-Ghani, A., Abou-Hashem, M. APPLICATION OF IMMUNOENZYMATIC TECHNIQUE IN DETERMINATION OF GALANTHAMINE IN TWO PANCRATIUM SPECIES. test, 1996; 5(2): 99-104. doi: 10.21608/zjps.1996.185026

APPLICATION OF IMMUNOENZYMATIC TECHNIQUE IN DETERMINATION OF GALANTHAMINE IN TWO PANCRATIUM SPECIES

Zagazig Journal of Pharmaceutical Sciences
Article 15, Volume 5, Issue 2, December 1996, Pages 99-104    PDF (3.16 M)
Document Type: Original Article
DOI: 10.21608/zjps.1996.185026
Authors
Taha Sarg* 1; Meinhart Zenk2; Sameih El-Dahmy1; Afaf Abdel-Ghani3; Maged Abou-Hashem4
1Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Egypt.
2Institute of Pharmaceutical Biology, Munich University, Munich, Germany
3Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Zagazig, Egypt.
4Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
Abstract
Enzyme-Linked Immunosorbent Assay (ELISA) was established to qualitatively and quantitatively determine galanthamine in the alcoholic extract of Pancratium foetidum Pomel bulbs and in the callus culture of P. arabicum Sick. The amount of galanthamine was 168 ng/g dry weight of bulbs and 314 ng/g dry weight of the callus. The measuring range for galanthamine extended from 0.1 – 100 ng per assay. The least amount to be detected was 0.01 ng and the recovery was 98 ±1.8 % .
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