Comparison of Various Bioactive Compounds in Leaves and Seeds of Tribulus longipetalus Viv. | ||
| Catrina: The International Journal of Environmental Sciences | ||
| Article 3, Volume 2, Issue 2, 2007, Pages 119-123 PDF (236.36 K) | ||
| Document Type: Original Article | ||
| Author | ||
| Inas Mohamed* | ||
| Medicinal and Aromatic Plants Department, Desert Research Center, El-Mataria, Cairo, Egypt | ||
| Abstract | ||
| A study was conducted to analyze different bioactive compounds in Leaves and seeds of Tribulus longipetalus include saponins, total proteins, amino acids, fat and flavonoids with one and two dimensional, thin layer and column chromatography followed by spectrophotometric analysis. Results indicate that leaves contained high concentrations of flavonoids and fat. Whereas levels of saponins, proteins, amino acids, total minerals and other organic compounds were high in seeds. The analysis of leaves and seeds of Tribulus longipetalus for these valuable organic compounds will provide important raw materials that can be used for preparation of medicines in pharmaceuticals companies. A single subcutaneous dose of CCl4 (5.93ml kg*1b.wt. which represents 24h LD100) severed as a toxicant, was used in this study. The results showed that ethyl acetate fraction was the most active fraction, then butanol, aqueous and dichloromethane fraction. By subjecting the ethyl acetate fraction to column chromatography it afforded fractions which are effective in reducing CCl4 induced mortalities. The efficiency of the isolated glycosides in reducing CCl4 –induced mortalities and some disturbances in biochemical parameters (liver transaminases AST, ALT and creatine kinas CK activities, serum urea and serum creatinine) were investigated in adult female Bufo regularis (Egyptian toads). The biochemical studies confirmed the bioassay studies. This suggestes that these saponin glycosides may be promoting in modulating CCl4- induced lethality and most of its toxic effects. | ||
| Keywords | ||
| Tribulus longipetalus; medicinal plants; Chemical analysis; biochemical activity | ||
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