BIOCHEMICAL STUDIES AND PARTIAL PURIFICATION OF CELLULASE ENZYME BIOSYNTHESIZED BY TRICHODERMA VIRIDE AND FUSARIUM OXYSPORIUM | ||
| Zagazig Journal of Pharmaceutical Sciences | ||
| Article 8, Volume 3, Issue 2, December 1994, Pages 69-77 PDF (4.96 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjps.1994.186481 | ||
| Authors | ||
| Mostafa El-Sayed* 1; Abdel-Fatah El-Essawy2; Mohamed Ghaly2; Samia Abd-El-Salam2 | ||
| 1Department of Botany, Faculty of Science, Tanta University, Egypt. | ||
| 2Department of Botany, Faculty of Science, Zagazig University, Egypt | ||
| Abstract | ||
| Precipitation of carboxymethyl cellulase enzyme biosynthesized by Trichoderma viride and Fusarium oxysporium was affected using organic solvents such as ethyl alcohol, acetone and butanol. Best results were obtained when 66% ethyl alcohol was used as a solvent. The precipitate was applied on a column chromatography with sephadex G-150 for fractionation and purification and the most active fractions were then collected. The optimal biochemical conditions under which the partially purified carboxy methyl cellulase (CMCase) exhibited its maximal activities were incubation temperature 50°C for 45 minutes at pH 5.0 in presence of 1% carboxy methyl cellulose. Zinc sulphate and calcium chloride were used as stimulators for the enzyme. | ||
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