Enteroviral infections in cases of miscarriage | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 8, Volume 11, Issue 1, April 2003, Pages 90-100 PDF (474.13 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejhm.2003.18721 | ||
| Authors | ||
| Eman A. Mohamed; Daad F.I. El Fouhil; Soad E. Abdel Rehim; Jaylan A. Ali; Salwa E. Abdel Hamid | ||
| Microbiology Department, Faculty of Medicine for Girls, Al–Azhar University | ||
| Abstract | ||
| To investigate enteroviral infections as possible causes of miscarriage in pregnant women, 100 women with miscarriage were studied, 75 of whom had miscarriage before the 13th week of gestation (group A) and 25 had miscarriage after the 13th week of gestation (group B). Placental tissue samples were taken from all women and were investigated for the presence of enteroviruses by cell culture in Vero cell line. The viral isolates of cell culture positive samples were subjected to neutralization test for typing, and to reverse transcription – polymerase chain reaction (RT–PCR) for detection of enteroviral RNA. It was found that 36 out of 100 placental tissue samples (36%) were cell culture positive as they showed enterovirus – like cytopathic effect (CPE) in Vero cell culture, including 29/75 samples (38.7%) from group A and 7/25 samples (28%) from group B, with statistically insignificant difference in results between both groups (P>0.05). All the 36 viral isolates of the cell culture positive samples were untypable by neutralization test with the available enteroviral antisera, while 26 of these isolates (72.2%) were positive for enteroviral RNA by RT–PCR, comprising 21/29 isolates (72.4%) from group A and 5/7 isolates (71.4%) from group B, with no statistically significant difference in results between both groups (P>0.05). The remaining 10 viral isolates were negative for enteroviral RNA by RT–PCR and were attributed to other CPE producing viruses which may induce miscarriage. As 26 out of 100 samples (26%) were positive in both cell culture and RT–PCR, thus the obtained incidence of enteroviral infections among cases of miscarriage was 26%. This incidence is considered to be a quite high one, needing further attention and studies. No relation was found between age or parity and enteroviral infections in the studied groups. Enteroviruses may be considered as important causative agents of miscarriage, and enteroviral infections should be investigated in all cases of miscarriage. Further studies including typing of enteroviral isolates from cases of miscarriage by sequencing are recommended. | ||
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