A,A, O., M.A El Tawel, F., H. Tawfik, Y. (2007). Regeneration and Micropropagation Systems of (Stevia rebaudiana) var. sponti. test, 28(JULY- SEPTEMBER), 116-121. doi: 10.21608/asejaiqjsae.2007.1878
Ouf A,A; Faiza M.A El Tawel; Yusreya H. Tawfik. "Regeneration and Micropropagation Systems of (Stevia rebaudiana) var. sponti". test, 28, JULY- SEPTEMBER, 2007, 116-121. doi: 10.21608/asejaiqjsae.2007.1878
A,A, O., M.A El Tawel, F., H. Tawfik, Y. (2007). 'Regeneration and Micropropagation Systems of (Stevia rebaudiana) var. sponti', test, 28(JULY- SEPTEMBER), pp. 116-121. doi: 10.21608/asejaiqjsae.2007.1878
A,A, O., M.A El Tawel, F., H. Tawfik, Y. Regeneration and Micropropagation Systems of (Stevia rebaudiana) var. sponti. test, 2007; 28(JULY- SEPTEMBER): 116-121. doi: 10.21608/asejaiqjsae.2007.1878

Regeneration and Micropropagation Systems of (Stevia rebaudiana) var. sponti

Alexandria Science Exchange Journal
Article 4, Volume 28, JULY- SEPTEMBER - Serial Number 3, July 2007, Pages 116-121    PDF (617.48 K)
Document Type: Original Article
DOI: 10.21608/asejaiqjsae.2007.1878
Authors
Ouf A,A1; Faiza M.A El Tawel2; Yusreya H. Tawfik3
1Laboratory of Biotechnology. Sugar crops Res. Inst., Agric. Res Center.ac. Agric., Saba basha, Alex. Univ.
2Plant Physiology Department. Sugar crops Res. Inst., Agric. Res. Center
3Plant Breeding Department , Sugar crops Res. Inst., Agric. Res. Center
Abstract
In order to establish an effective regeneration system of Stevia (Stevia rebaudiana) var. sponti, different concentrations of phytohormones were added to MS basal medium individually or in combination with (IAA, BAP and NAA) as callus induction media. The obtaining results indicated that MS basal medium which containing 0.5 mg/l of NAA and 0.5 BAP was the most efficient and effective medium for callus induction.
For shoot regeneration, different recombination of phytohormones were used, addition of NAA (0.1 mg/l) and BAP (1.0 mg/l) to MS basal medium under light condition caused high increase in shooted calli percentage.
Vigor root system was developed after the addition of 3 mg/l of IBA to MS basal medium, while the addition of high concentration of sucrose (80 g ∕ L) found to be ineffective factor for root regeneration and growth. Micropropagation system was performed by exposure of axillaries and terminal buds to solid MS medium which contained 0.1 IAA and 1.0 BA. Then, clusters were cut into small number of plantlets in MS liquid media without any hormone. This method was found to be more effective than using hormonal medium for micropropagation. 
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