TRIALS FOR PREPARATION OF INACTIVATED EGG DROP SYNDROME VIRUS VACCINE ADAPTED ON SPECIFIC PATHOGEN FREE EMBRYONATED CHICKEN EGGS (SPF-ECE) | ||
| Egyptian Journal of Agricultural Research | ||
| Article 46, Volume 88, Issue 3, September 2010, Pages 951-959 PDF (2.31 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2010.189418 | ||
| Authors | ||
| NADIA M. IBRAHIM; EMAN A. HASSAN; ABO-ZIED A. ABO-ZIED | ||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt. | ||
| Abstract | ||
| Egg drop syndrome — 76 (EDS-76) virus was propagated for three passages on embryonated duck eggs followed by thirty passages on C.E.F cell culture, then, for ten passages on SPF embryonated chicken eggs. The titer and HA activity of the 10th passage was 1095/ml and 210/m1 successively. Inactivation of passage number 10 of propagated virus on SPF-ECE with formaline was adjuvanted with paraffin oil. The prepared vaccine was compared with inactivated oil emulsion EDS76 prepared on embryonated duck eggs for sterility, safety and potency. The potency test was performed by measuring the cellular and humoral immune response. The efficiency of the prepared vaccine was estimated up to six months. | ||
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