Isolation and Identification of the Most Common Bacteria Isolated from Intestine of Broiler Chickens in Egypt | ||
| Journal of Applied Veterinary Sciences | ||
| Article 4, Volume 6, Issue 4, October 2021, Pages 23-27 PDF (524.16 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2021.87134.1092 | ||
| Authors | ||
| Eman M. Desouky1; Heba N. Deif* 2; Jakeen K. Eljakee2 | ||
| 1Master student at Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||
| 2Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||
| Abstract | ||
| Bacteria are seriously affecting broiler chickens and the poultry industry in many countries, as well as in Egypt. Both pathogenic and nonpathogenic bacterial species have been reported in broiler chickens. The present study aimed to isolate and identify the most common bacteria recovered from apparently healthy broiler chickens by conventional methods and the VITEK system. Samples were taken from 30 broiler chickens of different ages (3 – 5 weeks), from different retail markets in Cairo, during the period from January to August 2019. Bacteriological examination of the samples showed both Gram positive and Gram negative bacteria as well. Lysinibacillus sphaericus (n=7), Escherichia coli (n = 5), Proteus species (n = 4), Bacillus cereus (n = 3), with isolation rates of 23.3 %, 16.6 %, 13.3 % and 10 %, were identified. In addition to its pathogenicity and hazardous action, some strains of Lysinibacillus sphaericus have a larvicide effect on some species of mosquitoes. So, further studies and investigations will be conducted to test Lysinibacillus sphaericus and Bacillus cereus isolates for their biological control activity and / or their potency to remove, control, or reduce petroleum hydrocarbon soil contaminants in different localities. It will be used as an alternative control agent for numerous synthetic commercial formulations. | ||
| Keywords | ||
| B.cereus; Broilers; E.coli; L.sphaericus; Proteus species | ||
| References | ||
|
ADAMS, M. R. AND MOSS, M.O., 2005. Food Microbiology, 2nd Edition. The Royal Society of Chemistry, Thomas Graham House, Science Park, Milton Road, Cambridge CB4 OWF, UK; pp: 187-190.
AGUIRRE-MONROY, A. M., SANTANA-MARTÍNEZ, J. C. AND DUSSÁN, J., 2019. Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil replantation, Hindawi, Article ID 3075153. https://doi.org/10.1155/2019/3075153
BARBOSA, T. M., SERRA, C. R., LA RAGIONE R. M., WOODWARD M .J. AND HENRIQUES A.O., 2005. Screening for Bacillus isolates in the broiler gastrointestinal tract. Appl. Environ. Microbiol 71(2): 968-978.
BARROW, G. I. AND FELTHAN, R. K. A., 2004. Cowan and Steels identification of Medical bacteria. 4th Edition. Cambridge University Press 50-145.
BUSHEN, A., TEKALIGN, E. AND ABAYNEH, M., 2021. Drug- and Multidrug-Resistance Pattern of Enterobacteriaceae Isolated from Droppings of Healthy Chickens on a Poultry Farm in Southwest Ethiopia. Infection and Drug Resistance 14: 2051-2058.
CAMPBELL, N. A., REECE, J. B., 2002. Biology, Pearson Education Inc, San Francisco.
CLAVIJO, V. AND FLOREZ, M. J. V., 2018. The gastrointestinal microbiome and its association with the control of pathogens in broiler chicken production: a review. Poult. Sci 97:1006-1021.
COLIN, B., 2012. The bacterium, Lysinibacillus sphaericus, as an insect pathogen. Journal of Invertebrate Pathology 109 (1): 1–10.
DANDACHI, I., SOKHN, E. S., DAHDOUH, E. A., AZAR, E., EL-BAZZAL, B., ROLAIN, J. AND DAOUD, Z., 2018. Prevalence and Characterization of Multi-Drug-Resistant Gram-Negative Bacilli Isolated From Lebanese Poultry: A Nationwide Study. Front Microbiol 9: 550.
DUC, L. H., HONG, H. A., BARBOSA, T. M., HENRIQUES, A. O. AND CUTTING, S. M., 2004. Characterisation of Bacillus probiotics available for human use. Appl. Environ. Microbiol 70:2161-2171.
FORGHANI, F., KIM, J. B. AND OH, D. H., 2014. Enterotoxigenic profiling of emetic toxin- and enterotoxin-producing Bacillus cereus, isolated from food, environmental, and clinical samples by multiplex PCR. Journal of Food Science 79: 2288-2293. https://doi.org/10.1111/1750-3841.12666 FSANZ 2018.
GREEN, D. H., WAKELEY, P. R., PAGE, A., BARNES, A., BACCIGALUPI, L., RICCA, E. AND CUTTING, S. M., 1999. Characterization of two Bacillus probiotics. Appl. Environ. Microbiol 65:4288-4291.
HAQUE, M. A., QUAN, H., ZUO, Z., KHAN, A., SIDDIQUE, N. AND HE, C., 2021. Pathogenicity of feed-borne Bacillus cereus and its implication on food safety. Agrobiological Records 3: 1-16.
HOA, N. T., BACCIGALUPI, L., HUXHAM, A., SMERTENKO, A., VAN, P. H., AMMENDOLA, S., RICCA, E. AND CUTTING, A. S., 2000. Characterization of Bacillus species used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders. Appl. Environ. Microbiol 66:5241-5247.
HOLT, J. G., KRIEG, N. R. AND SNEATH, P. H. A., 1994. Bergey’s manual of determinative bacteriology, 9th edn. Baltimore: Williams and W Wilkins.
HÖLZEL, C. S., TETENS, J. L. AND SCHWAIGER, K., 2018. Unraveling the Role of Vegetables in Spreading Antimicrobial-Resistant Bacteria: A Need for Quantitative Risk Assessment. Foodborne Pathog Dis 15(11):671-688.
HOSSAIN, M. T., SIDDIQU, M. P., HOSSAIN, F. M. A., ZINNAH, M. A., HOSSAI, M. M., ALAM, M. K., RAHMAN, M. T. AND CHOUDHURY, K. A., 2008. Isolation, identification, toxin profile and antibiogram of Escherichia coli isolated from broilers and layers in mymensingh district of Bangladesh. Bangl. J. Vet. Med 6 (1): 01-05.
IFTIKHAR, A., AKIRA, Y., ATSUSHI, Y. AND TORU, F., 2007. Proposal of Lysinibacillus boron tolerant gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. Nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. Nov". International Journal of Systematic and Evolutionary Microbiology 57 (5): 1117-1125.
JENSEN, G. B., HANSEN, B. M., EILENBERG, J. AND MAHILLON, J., 2003. The hidden lifestyles of Bacillus cereus and relatives. Environ Microbiol 5: 631-640.
MARTÍNEZ, S. A. AND DUSSÁN, J., 2017. “Lysinibacillus sphaericus plant growth promoter bacteria and lead phytoremediation enhancer with Canavalia ensiformis,” Environmental Progress & Sustainable Energy, vol. 37, no. 1, pp. 276–282.
MAY, F. J., POLKINGHORNE, B. G. AND FEARNLEY, E. J., 2016. Epidemiology of bacterial toxin-mediated food-borne gastroenteritis outbreaks in Australia, 2001 to 2013. Commun Dis Intell Q Rep. 40(4):E460-E469.
NGUYEN, A. T. AND TALLENT, S. M., 2019. Screening food for Bacillus cereus toxins using whole-genome sequencing. Food Microbiol 78:164-170.
NICHOLSON, W. L. AND SETLOW, P., 1990. Sporulation, germination and outgrowth, p. 391-450. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons Ltd., Chichester, United Kingdom.
O’DRISCOLL, J., 1977. Venereal Infection in thoroughbreds with bacillus Proteus mirabilis. Veterinary Record 100: 534.
PATTERSON, J. A. AND BURKHOLDER, K. M., 2003. Application of prebiotics and probiotics in poultry production. Poult. Sci 82:627-631.
PINE, J. H., RITCHER, W. R. AND ESTERLY, J. R., 1973. Bacterial Pneumonia: ultrastructural, autoradiographic and histochemical observations. American Journal of Pathology 73: 115-124.
QUINN, P. J., MARKEY, B. K., CARTER, M. E., DONNELLY, W. J. AND LEONARD, F. C., 2002. Veterinary Microbiology and Microbial Disease.1st Edition.Cornwall, Great Britain: Blackwell Science Ltd. 43-122.
RANJITKAR, S.,LAWLEY, B., TANNOCK, G. AND ENGBERG, R. M., 2016. Bacterial Succession in the Broiler Gastrointestinal Tract Appl Environ Microbiol 82(8): 2399-2410.
ROY, S. R., RAHMAN, M. B., HASSAN, J. AND NAZIR, N.H., 2012. Isolation and Identification of Bacterial Flora from Internal Organs of Broiler and Their Antibiogram Studies. Microbes and Health 1(2):72-75.
SALYERS, A. A. AND WHITT, D. D., 2002. Bacterial pathogenesis: a molecular approach, ASM Press, Washington DC.
STECHER, B. AND HARDT, W., 2008. The role of microbiota in infectious disease. Trends Microbiol; 16: 107-114.
THEIN, C. C., TRINIDAD, R. M. AND PAVLIN, B. I., 2010. A large food borne outbreak on a small Pacific island. Pac Health Dialog 16(1):75-80.
WEI, S., MORRISON, M. AND YU, Z., 2013. Bacterial census of poultry intestinal microbiome. Poult Sci Sympos 92:671-83.
WILSON, G.S. AND MILES, A., 1975. Principles of Bacteriology, virology and immunity.6th Edition. London: Edward Arnold Ltd 887-900.
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