Sh. Osman, A., H. Abdel Wahed, M., S. Tolba, M., K. A. Khalafallah, K. (2016). BROCCOLI GROWTH, ANTIOXIDANT ACTIVITY AND HEALTH TISSUES RESPONSES TO ASCORBIC ACID UNDER DEFICIT IRRIGATION. test, 30(2), 106-133. doi: 10.21608/fjard.2016.191751
Ashraf Sh. Osman; Mohamed H. Abdel Wahed; Mofreh S. Tolba; Kareem K. A. Khalafallah. "BROCCOLI GROWTH, ANTIOXIDANT ACTIVITY AND HEALTH TISSUES RESPONSES TO ASCORBIC ACID UNDER DEFICIT IRRIGATION". test, 30, 2, 2016, 106-133. doi: 10.21608/fjard.2016.191751
Sh. Osman, A., H. Abdel Wahed, M., S. Tolba, M., K. A. Khalafallah, K. (2016). 'BROCCOLI GROWTH, ANTIOXIDANT ACTIVITY AND HEALTH TISSUES RESPONSES TO ASCORBIC ACID UNDER DEFICIT IRRIGATION', test, 30(2), pp. 106-133. doi: 10.21608/fjard.2016.191751
Sh. Osman, A., H. Abdel Wahed, M., S. Tolba, M., K. A. Khalafallah, K. BROCCOLI GROWTH, ANTIOXIDANT ACTIVITY AND HEALTH TISSUES RESPONSES TO ASCORBIC ACID UNDER DEFICIT IRRIGATION. test, 2016; 30(2): 106-133. doi: 10.21608/fjard.2016.191751

BROCCOLI GROWTH, ANTIOXIDANT ACTIVITY AND HEALTH TISSUES RESPONSES TO ASCORBIC ACID UNDER DEFICIT IRRIGATION

Fayoum Journal of Agricultural Research and Development
Article 8, Volume 30, Issue 2, July 2016, Pages 106-133    PDF (1.48 M)
Document Type: Research articles.
DOI: 10.21608/fjard.2016.191751
Authors
Ashraf Sh. Osman1; Mohamed H. Abdel Wahed2; Mofreh S. Tolba1; Kareem K. A. Khalafallah1
1Horticulture Department, Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt
2Soils and Water Department (Agric. Eng.), Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt
Abstract
Two field experiments were conducted during 2013/2014 and
2014/2015 in a private Farm, Ibshway district, Fayoum, Egypt to study deficit
irrigation (60, 80 and 100% ETc) and ascorbic acid foliar application (0, 200
and 400 mg l-1) on growth, relative water content (RWC), membrane stability
index (MSI), electrolyte leakage (EL), leaf photosynthetic pigments (LPS),
osmoprotectants (OS) and DPPH radical-scavenging activity of broccoli
plants. The obtained results clarified that, irrigation amount at 80% or 100%
ETc, significantly, resulted in higher mean values of stem length and
diameter, number of leaves plant-1, leaf MSI, RWC, carotenoids, DPPHradical-scavenging and total free amino acids content than irrigation amount
at 60% ETc. On other side, irrigation amounts, irrespective the level used, did
not reflect any noticeable impact on number of branches plant-1 and head
contents of ascorbic acid. Increasing irrigation water from 60% up to 100%
ETc decreased the contents of leaf EL, chlorophyll A, B, A + B, anthocyanin,
free proline and TSS. Exogenous application of ascorbic acid at
concentrations of 200 and 400 mg l-1, significantly improved most studied
growth parameters, RWC, MSI, anthocyanin, DPPH, total free amino acids
and endogenous ascorbic acid however, leaf EL decreased gradually.
Moreover, the impact of ascorbic acid, irrespective of the concentration used,
on leaf photosynthetic pigment, free proline and total soluble sugars contents
was at par.

Keywords
Deficit irrigation; Ascorbic acid; Antioxidant activity; Broccoli growth; RWC; MIS; EL; leaf photosynthetic pigments; osmoprotectants and DPPH radical-scavenging activity
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